Does anyone know which mutations the cell lines Huh7, Hep G2, Hep 3b have?

More Rafael Ioris's questions See All

Reagents and protocol for transient transfection in meduloblastoma cell lines?

does anyone can suggest reagents and protocols for transient transfection (pcDNA 3.1 plasmid) in meduloblastoma cell lines (daoy, d283, d425, d458 and hd-mb03)? We normally use PEI for transient...

06 July 2019 9,075 2 View

Anyone using BIOCOLOR life science assays?

We recently bought 3 kit to measure collagen and elastin in cells... The manuals are very confusing, badly written with sparse informations... But the worst is to contact costumer/technical...

08 September 2018 3,056 3 View

How to remove non-tumour cells from a mouse tumour?

We have a breast cancer mouse model from which we want to extract the tumour, dissociate in single cells and then remove all non-tumour cells to have a pure population of tumour cells to inject in...

09 October 2016 2,298 2 View

Can someone recommended antibodies to perform a co-staining (IHC-P) of CD44/CD24 in paraffin embedded sections (mouse samples)?

Is it possible to do a co-staining in paraffin embedded section with CD44/CD24 antibodies? Mouse samples... thanks

07 August 2016 8,806 2 View

Does anyone added NAD+ (or any precursor) in culture media?

I am working with human cancer cell lines and the goal is to increase NAD+ content in the cells. Nonetheless, I have no idea about the concentration that I should add, if NAD+ (or any precursor)...

02 March 2016 7,623 3 View

Does anyone added citrate and/or pyruvate in culture media?

I am working with human cancer cell lines and the goal is to add sodium citrate and/or sodium pyruvate to the culture media. Nonetheless, I have no idea about the concentration that I should...

01 February 2016 9,568 4 View

Did anyone used an inducible system that express rtTA ubiquitously in transgenic mice?

We are engaged in a metabolic project where we want to express our gene in a whole body and inducible fashion (or at least in important metabolic tissues as muscle, WAT, BAT and liver). Do anyone...

31 December 2014 5,376 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Ke-Wei Zhao

Perhaps a RG paper may help answer your question:

https://www.researchgate.net/publication/51213593_Low_Frequency_of_PIK3CA_Gene_Mutations_in_Hepatocellular_Carcinoma_in_Chinese_Population

Article Low Frequency of PIK3CA Gene Mutations in Hepatocellular Car...

Didier Fesquet

data only available for huh7

https://cansar.icr.ac.uk/cansar/cell-lines/Huh-7/