Reagents and protocol for transient transfection in meduloblastoma cell lines?

does anyone can suggest reagents and protocols for transient transfection (pcDNA 3.1 plasmid) in meduloblastoma cell lines (daoy, d283, d425, d458 and hd-mb03)? We normally use PEI for transient...

06 July 2019 8,947 2 View

Anyone using BIOCOLOR life science assays?

We recently bought 3 kit to measure collagen and elastin in cells... The manuals are very confusing, badly written with sparse informations... But the worst is to contact costumer/technical...

08 September 2018 2,927 3 View

How to remove non-tumour cells from a mouse tumour?

We have a breast cancer mouse model from which we want to extract the tumour, dissociate in single cells and then remove all non-tumour cells to have a pure population of tumour cells to inject in...

09 October 2016 2,155 2 View

Can someone recommended antibodies to perform a co-staining (IHC-P) of CD44/CD24 in paraffin embedded sections (mouse samples)?

07 August 2016 8,613 2 View

Does anyone added NAD+ (or any precursor) in culture media?

02 March 2016 7,507 3 View

Does anyone added citrate and/or pyruvate in culture media?

I am working with human cancer cell lines and the goal is to add sodium citrate and/or sodium pyruvate to the culture media. Nonetheless, I have no idea about the concentration that I should...

01 February 2016 9,443 4 View

Did anyone used an inducible system that express rtTA ubiquitously in transgenic mice?

We are engaged in a metabolic project where we want to express our gene in a whole body and inducible fashion (or at least in important metabolic tissues as muscle, WAT, BAT and liver). Do anyone...

31 December 2014 5,256 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View