Why qPCR is showing such curve instead of an perfect one on the following picture ?

Dear all, I wonder if you could help me with the interpretation of such charts I get sometimes when I do amplification of covid19 by using ORF gene ( please see the picture i attached the graph...

07 June 2020 7,656 3 View

How to measure accurate specific heat capacity with DSC?

I am using Mettler Toledo DSC 2 to find cp of In/Zn with sapphire and direct methods. The DSC is calibrated with both In and Zn and is within the specifications. However when I try to find cp...

16 September 2019 9,331 8 View

Please guide, which gene primers are used for quantification analysis of cell free and circulating tumor DNA gene ?

I am to find out the quantification of circulating tumor DNA and cell free DNA through real time PCR, for that purpose in PCR which gene primers should be included for amplification

28 August 2019 5,538 4 View

What is the difference between Group of Pictures (GOP) and video?

I am currently working on video transcoding. I was going through literature and I saw that the authors of that literature have considered group of pictures for transcoding rather than video. Is...

21 August 2018 6,868 3 View

Possibility to get sequence information of primers premixed in a ready to use PCR kit/master mix?

03 September 2017 1,559 3 View

Expression of penta-peptide in a suitable Mammalian expression vector?

Hi I need to express a 5 AA long peptide in any suitable mammalian expression vector. CI fear that if its allowed to be expressed in pCDNA3.1, there might be a leaky overhangs of extra amino acids...

21 March 2017 7,432 3 View

Future of electronics will soon depend on graphene nanoribbons. Anyone know how to control the ribbons growth and their alignment?

Graphene nano-ribbons can be grown directly on the surface of a semiconductor, but it's hard to control the ribbons growth, and at the same time, aligning them all in the same direction.

06 February 2016 144 2 View

How can I perform Search and Filtration Funcationalities on Database with some Knowledgebase?

I have a database with millions of records stored in it. I want to implement Search and Functionalists in My db with some AI and data mining techniques and methods. Suggest me Some Algo, Methods...

04 December 2014 7,605 2 View

Is there a good antibody available to detect C-terminal fragments of Amyloid Precursor Protein (695 AA isoform)?

I am trying to quantify the levels of C-terminal fragments of APP, generated after BACE1 cleavage in APP/swAPP cell lines (in different experimental conditions). I have tried some commercially...

26 June 2014 5,572 7 View

If I would like to venture into the research of bullet-proof vests. What would be the best possible materials to use?

At present, bullet-proof vests contain many layers of tightly-woven fabric such as Kevlar (or hard, strong materials such as ceramic or titanium) which can stop the metal pistol or revolver...

15 April 2014 6,222 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View