Why qPCR is showing such curve instead of an perfect one on the following picture ?

Dear all, I wonder if you could help me with the interpretation of such charts I get sometimes when I do amplification of covid19 by using ORF gene ( please see the picture i attached the graph...

07 June 2020 7,656 3 View

How to measure accurate specific heat capacity with DSC?

I am using Mettler Toledo DSC 2 to find cp of In/Zn with sapphire and direct methods. The DSC is calibrated with both In and Zn and is within the specifications. However when I try to find cp...

16 September 2019 9,331 8 View

Please guide, which gene primers are used for quantification analysis of cell free and circulating tumor DNA gene ?

I am to find out the quantification of circulating tumor DNA and cell free DNA through real time PCR, for that purpose in PCR which gene primers should be included for amplification

28 August 2019 5,538 4 View

What is the difference between Group of Pictures (GOP) and video?

I am currently working on video transcoding. I was going through literature and I saw that the authors of that literature have considered group of pictures for transcoding rather than video. Is...

21 August 2018 6,868 3 View

Possibility to get sequence information of primers premixed in a ready to use PCR kit/master mix?

03 September 2017 1,559 3 View

Expression of penta-peptide in a suitable Mammalian expression vector?

Hi I need to express a 5 AA long peptide in any suitable mammalian expression vector. CI fear that if its allowed to be expressed in pCDNA3.1, there might be a leaky overhangs of extra amino acids...

21 March 2017 7,432 3 View

Future of electronics will soon depend on graphene nanoribbons. Anyone know how to control the ribbons growth and their alignment?

Graphene nano-ribbons can be grown directly on the surface of a semiconductor, but it's hard to control the ribbons growth, and at the same time, aligning them all in the same direction.

06 February 2016 144 2 View

Does anybody have experience in gene sequencing through HRM?

A.o.A , Please let me know if any one has used High Resolution melting analysis technique for mutation analysis. If yes than please which type of Real Time PCR is used. What other accessories...

01 February 2015 1,635 1 View

How can I perform Search and Filtration Funcationalities on Database with some Knowledgebase?

I have a database with millions of records stored in it. I want to implement Search and Functionalists in My db with some AI and data mining techniques and methods. Suggest me Some Algo, Methods...

04 December 2014 7,605 2 View

Is there a good antibody available to detect C-terminal fragments of Amyloid Precursor Protein (695 AA isoform)?

I am trying to quantify the levels of C-terminal fragments of APP, generated after BACE1 cleavage in APP/swAPP cell lines (in different experimental conditions). I have tried some commercially...

26 June 2014 5,572 7 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View