Asiyah Bukhari

4 Questions 1 Answers 0 Followers

Questions related from Asiyah Bukhari

Protocol for quantification of Cell free DnA on qubit flourometer?

I am working on Cell free DNA extraction from breast cancer patients. protocol was optimized. I wanted to quantify the cell free DnA extracted from the plasma . Kit used on qubit was Qubit dsDNA...

02 September 2020 6,180 1 View

Anyone of you have used Epiquik Circulating Cell Free DNA Isolation Easy Kit with EPimag HT 96 well magnetic seperator ?

Dear Researcher , can you please tell me that how is effectively using this kit mentioned above , i have procured this and trying to optimize the protocol but finding problem in DNA extraction in...

14 November 2019 2,764 0 View

Please guide, which gene primers are used for quantification analysis of cell free and circulating tumor DNA gene ?

I am to find out the quantification of circulating tumor DNA and cell free DNA through real time PCR, for that purpose in PCR which gene primers should be included for amplification

29 August 2019 5,618 4 View

Does anybody have experience in gene sequencing through HRM?

A.o.A , Please let me know if any one has used High Resolution melting analysis technique for mutation analysis. If yes than please which type of Real Time PCR is used. What other accessories...

02 February 2015 1,705 1 View