Why qPCR is showing such curve instead of an perfect one on the following picture ?

Dear all, I wonder if you could help me with the interpretation of such charts I get sometimes when I do amplification of covid19 by using ORF gene ( please see the picture i attached the graph...

07 June 2020 7,656 3 View

How to measure accurate specific heat capacity with DSC?

I am using Mettler Toledo DSC 2 to find cp of In/Zn with sapphire and direct methods. The DSC is calibrated with both In and Zn and is within the specifications. However when I try to find cp...

16 September 2019 9,331 8 View

Please guide, which gene primers are used for quantification analysis of cell free and circulating tumor DNA gene ?

I am to find out the quantification of circulating tumor DNA and cell free DNA through real time PCR, for that purpose in PCR which gene primers should be included for amplification

28 August 2019 5,538 4 View

What is the difference between Group of Pictures (GOP) and video?

I am currently working on video transcoding. I was going through literature and I saw that the authors of that literature have considered group of pictures for transcoding rather than video. Is...

21 August 2018 6,868 3 View

How to calculate true positive(TP), TN, and FN of for an image where i am trying to compare with KM and FCM clustering with my proposed method?

i think the formula which can be used is sensitivity =(TP/TP+FN)*100.But i wanna know how to take values of TP or etc of an image?

23 July 2018 8,992 3 View

What's new can be done in Digital Image Watermarking using LSB?

24 September 2017 6,625 7 View

Possibility to get sequence information of primers premixed in a ready to use PCR kit/master mix?

03 September 2017 1,559 3 View

Expression of penta-peptide in a suitable Mammalian expression vector?

Hi I need to express a 5 AA long peptide in any suitable mammalian expression vector. CI fear that if its allowed to be expressed in pCDNA3.1, there might be a leaky overhangs of extra amino acids...

21 March 2017 7,432 3 View

Future of electronics will soon depend on graphene nanoribbons. Anyone know how to control the ribbons growth and their alignment?

Graphene nano-ribbons can be grown directly on the surface of a semiconductor, but it's hard to control the ribbons growth, and at the same time, aligning them all in the same direction.

06 February 2016 144 2 View

Does anybody have experience in gene sequencing through HRM?

A.o.A , Please let me know if any one has used High Resolution melting analysis technique for mutation analysis. If yes than please which type of Real Time PCR is used. What other accessories...

01 February 2015 1,635 1 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Which regression is best fit when moderator is categorical and dependent variable is latent in nature?

I am using categorical variables as a moderator variable, and my dependent, mediator and IV variables are latent in nature. Which regression test I can apply. As logistic regression is for binary...

11 February 2021 1,584 3 View

ETA-squared statistics used to correlate observed versus measured variables?

Can the eta coefficient be used when the categorical and the quantitative variable is dependent? E.g. in the field, I made a prediction into a category (e.g. high, low or medium) of what I think a...

08 February 2021 3,756 3 View

What is the best way to encode categorical variables in datasets with many features and avoid increasing dimensionality of the dataset?

I am working with a large dataset that has many categorical variables with many classes in each variable. I want to encode the categorical variables without increasing the dimensionality of the...

31 January 2021 6,986 3 View

Is it possible to compare two unequal sample sizes with categorical data on SPSS?

I have a small dataset collected from SMEs and I need to compare the demographic characteristics between SMEs with access to external finance and SMEs who do not have access to finance on SPSS....

28 January 2021 2,503 4 View

Study Design for 3 Interval DVs, mixed IVs?

I'm conducting a study on mental health terminology on social media for a group project. We have three interval DVs measured through likert scales and three IVs. The IVs are social media usage...

28 January 2021 2,871 3 View

S Is ordered logit models can applied when the DV span from negative to positive values?

Trying to understand changes in public transit use following Covid-19, our categorical DV includes 5 values: 1 and 2 indicate that participants reduced their used (to a large & moderate extent...

26 January 2021 5,918 3 View

Which statistical test is suitable to test if 2 explanatory variables (different scales: ordinal & categorical) predict dependent variable (ordinal)?

I'm working on survey data that is inclusive of ordinal (1-5 rating scale), categorical (yes/no), nominal (frequency response). I have reduced many ordinal variables (those in ordinal scale) to 3...

26 January 2021 804 12 View

Should I run an ordinal regression, or a multiple regression?

Currently I am working to defend my thesis, and I ran into a technical question while analyzing my data: Should I conduct an ordinal or a multiple regression? My model is set up such that I have...

20 January 2021 2,104 3 View

By using SPSS program, Can I do generalized structural equation modelling?

By using SPSS program, Can I do generalized structural equation modelling? All predictors are categorical data and the outcome is also categorical

14 January 2021 5,064 3 View