Lakshmi Narayana Lavu

6 Questions 25 Answers 0 Followers

Questions related from Lakshmi Narayana Lavu

Fresh, decontaminated, hypo and UV treated wares and area being used as suggested. Still false positive appears in no template control, why?

I am using all the fresh working stocks, water, aerosol tips, changed the working room areas and further more decontaminated the working area with bleach and UV. Still than consistently false...

28 September 2015 2,827 3 View

Is there any commercial ELISA kit known to quantify gluten content in nutraceutical samples?

Please suggest any kit known to quantify gluten (Gliadin) in nutraceutical samples. So far I've used commercial ELISA kits to quantify gluten in food and raw seed samples. But this particular kit...

13 February 2015 6,351 3 View

Where can I get the certified DNA standard of Clostridium botulinum type A, B, E, F strains?

Can you suggest where can I get the certified DNA standard for Clostridium botulinum above said 4 types for PCR based screening of samples for botulism control?

03 February 2015 6,073 3 View

Can I replace the reporter dye without changing the probe sequence?

Hi, As a beginner of this I wanted to be clear about few things regarding fluorescent probes and their dyes. In one published document I selected below two probes for a duplex qPCR 1)....

04 November 2014 4,013 7 View

Are there any genus level detection primers for fish species?

Are there good working references to gene or it's primers set for detecting fish species (to cover most possible number of abundant fish species) in food samples using routine PCR?

04 November 2014 6,441 3 View

Multiple bands and laddering effect on agarose gel - any thoughts?

I am struggling with a serous problem in detection of GMO by routine PCR using CamvP35 and Nos amplification primers. This is a very serious problem I am facing since 4 months in our lab. Where I...

06 August 2014 4,931 9 View