Why qPCR is showing such curve instead of an perfect one on the following picture ?

Dear all, I wonder if you could help me with the interpretation of such charts I get sometimes when I do amplification of covid19 by using ORF gene ( please see the picture i attached the graph...

07 June 2020 7,656 3 View

How to measure accurate specific heat capacity with DSC?

I am using Mettler Toledo DSC 2 to find cp of In/Zn with sapphire and direct methods. The DSC is calibrated with both In and Zn and is within the specifications. However when I try to find cp...

16 September 2019 9,331 8 View

Please guide, which gene primers are used for quantification analysis of cell free and circulating tumor DNA gene ?

I am to find out the quantification of circulating tumor DNA and cell free DNA through real time PCR, for that purpose in PCR which gene primers should be included for amplification

28 August 2019 5,538 4 View

What is the difference between Group of Pictures (GOP) and video?

I am currently working on video transcoding. I was going through literature and I saw that the authors of that literature have considered group of pictures for transcoding rather than video. Is...

21 August 2018 6,868 3 View

Possibility to get sequence information of primers premixed in a ready to use PCR kit/master mix?

03 September 2017 1,559 3 View

Expression of penta-peptide in a suitable Mammalian expression vector?

Hi I need to express a 5 AA long peptide in any suitable mammalian expression vector. CI fear that if its allowed to be expressed in pCDNA3.1, there might be a leaky overhangs of extra amino acids...

21 March 2017 7,432 3 View

Future of electronics will soon depend on graphene nanoribbons. Anyone know how to control the ribbons growth and their alignment?

Graphene nano-ribbons can be grown directly on the surface of a semiconductor, but it's hard to control the ribbons growth, and at the same time, aligning them all in the same direction.

06 February 2016 144 2 View

Does anybody have experience in gene sequencing through HRM?

A.o.A , Please let me know if any one has used High Resolution melting analysis technique for mutation analysis. If yes than please which type of Real Time PCR is used. What other accessories...

01 February 2015 1,635 1 View

How can I perform Search and Filtration Funcationalities on Database with some Knowledgebase?

I have a database with millions of records stored in it. I want to implement Search and Functionalists in My db with some AI and data mining techniques and methods. Suggest me Some Algo, Methods...

04 December 2014 7,605 2 View

Is there a good antibody available to detect C-terminal fragments of Amyloid Precursor Protein (695 AA isoform)?

I am trying to quantify the levels of C-terminal fragments of APP, generated after BACE1 cleavage in APP/swAPP cell lines (in different experimental conditions). I have tried some commercially...

26 June 2014 5,572 7 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

When to use different framework?

Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...

18 February 2021 9,581 2 View

How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View

What does the value 0.498 in reliability?

There are 6 television programmes of different TV genres and their mean and mean ranks are put for each of them. But the reliability value of all the programmes together is 0.498 . So what exactly...

15 February 2021 7,686 4 View