Dear researchers
We are preparing for superresolution microscopy. I read some papers where the cells are prepared with the use of a special cytoskeletal buffer (CB) and followed by NaBH4 incubation. I have two questions.
1 What is the purpose of the CB [10 mM 2-(N-morpholino)ethanesulfonic acid, pH 6.1, 150 mM NaCl, 5 mM ethylene glycol tetraacetic acid, 5 mM MgCl2, 5 mM glucose]
2 Why is the NaBH4 incubation?
Thanks for an answer.