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Questions related from Patrick Corbin Mullen
I have been trying to use PCR to amplify cDNA using standard Taq polymerase. I am able to amplify beta actin, but not my target. Unfortunately, the Tm for my two primers is somewhat far apart...
24 January 2019 9,493 5 View
I have a curious result where my protein of interest is significantly down-regulated (90%) in knockout cells by Western blot, but appears to be unchanged on IF. I have repeated the western result...
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