Patrick Corbin Mullen

3 Questions 12 Answers 0 Followers

Questions related from Patrick Corbin Mullen

How does cycloheximide influence RT-PCR experiments?

I know that cycloheximide treatment prior to RT-PCR experiments is used to assess nonsense-mediated decay caused by premature stop codons. Generally if a mutation causes mRNA to be reduced at...

08 February 2019 4,033 0 View

How should a PCR annealing temperature gradient work?

I have been trying to use PCR to amplify cDNA using standard Taq polymerase. I am able to amplify beta actin, but not my target. Unfortunately, the Tm for my two primers is somewhat far apart...

24 January 2019 9,504 5 View

Why is signal for knockout cells drastically reduced (90%) on WB but not IF?

I have a curious result where my protein of interest is significantly down-regulated (90%) in knockout cells by Western blot, but appears to be unchanged on IF. I have repeated the western result...

18 January 2019 4,571 0 View