Duco Kramer

9 Questions 96 Answers 0 Followers

Questions related from Duco Kramer

Can serum decrease the IgG-binding capacity to Gamma beads?

Dear reader, Maybe you can help us out w/ an IP-problem that we stumble upon. We try to do an immuno precipitation of a protein (POI) that is expressed in skin. The Protein G SepharoseTM 4 Fast...

30 May 2025 4,427 2 View

Is there an alternative for LAS-software that works w/ Leica microscope camera?

We have a Leica microscope w/ a DFC350FX microscope camera attached, LAS 4.13 software to make the images. All works perfect, but now our institution is upgrading from Windows 10 to W11. From...

11 November 2024 1,585 3 View

Where to buy precast preparitive SDS-PAG gels?

Dear RG-community We wonder where we can buy commercial preparative gels for SDS-PAGE. This is a gel with one large slot for the sample and one or two small slots for kD-marker. We do use the...

25 May 2021 3,182 2 View

Is Leica LAS AF lite 4.x compatible with windows 10?

Dear resercher, Our institution will soon transit to windows 10 (finally ;) We use the LAS AF-lite 2.6 to view microscopic images collected from Leica machine (LAS X). With the transition to...

18 March 2021 2,022 3 View

Why use special cytoskeletal buffer for superresolution microscopy?

Dear researchers We are preparing for superresolution microscopy. I read some papers where the cells are prepared with the use of a special cytoskeletal buffer (CB) and followed by NaBH4...

21 February 2019 4,020 4 View

Do you know human nuclear marker protein?

Dear research helpers We are looking for a protein that is present in the nucleus of (each) human cells. The ideal protein is present with a few copies in the nucleus. Do you know of a protein...

14 February 2019 7,039 3 View

Can GEEM (gold enhance) be used to substitute silver in TEM-sample

We are planning to do a pre-embedding immunoreaction for EM. Although there are many alternatives we will try the DAB reaction on HRP-conjugated Ab, followed by silver precipitation (Gallyas,...

13 June 2017 8,584 7 View

What happened to the Alkaline ethanol and it turned dark brown?

After consulting a colleague I tried to produce sodium ethoxide by mixing NaOH in 96% EtOH. I tried to do so and ended up with a turbid white solution. An earlier question on RG revealed that I...

04 January 2016 9,855 7 View

How to clean formvar coated Cu or Ni grids for reuse?

I just realized that a grid for EM is quite expensive. Ni and Cu grids (one-hole in my case) cost around €0,5 each ($0,7). I have tried with overnight chloroform bu that was not satisfactory. Does...

11 December 2013 2,393 8 View