Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

Can someone explain blocking vs. nonblocking electrodes in terms of electrochemical impedance spectroscopy?

I have fabricated thin films and want to measure the impedance parallel to the material. I suspect the films are conducting protons and electrons based on some Hall effect measurements I have...

27 January 2021 1,712 5 View

Isoabsorbance and 3D Plots are missing under the "Spectra" toolbar of Agilent Chemstation 1200 series software. How can I retrieve it please?

The Problem started after replacing the HPLC Pump from Quaternary to Isocratic pump due to pump failure. The configuration was set as the previous set up as Modular 3D LC System and the license...

11 October 2020 8,018 3 View

I am using a rat TNFa ELISA to measure TNFa in culturing media. How soon following my alcohol treatment should I collect and measure TNFa?

I am using an organotypic hippocampal slice culture where we are exposing our slices to alcohol with subsequent alcohol withdrawal. I want to make sure I am capturing the right time to assess...

16 September 2020 7,020 2 View

When is simulation necessary in copula modeling?

When modeling dependencies using copulas, when is simulation necessary? Kindly need expert advice on this.

08 June 2020 7,166 3 View

Has anyone using organotypic hippocampal slice culture models observed holes in their tissue specimens between 6-12 days in vitro?

Our lab has noticed that after several tissue preparations for organotypic hippocampal slice cultures, some of our tissue is developing what appears to be large holes between the CA1 and dentate...

03 March 2020 3,981 3 View

How do I graph RT-PCR amplification values?

I have conducted an experiment to investigate the up-regulation and down-regulation of immune related genes. I have calculated the PCR efficiency and used the pfaffl method to get amplification...

22 October 2019 1,231 1 View

Is Learning Analytics under Data Mining?

In what ways EDM and LA differ and does LA under Data Mining and does it use the same techniques from Data Mining?

30 April 2019 8,370 1 View

Why use special cytoskeletal buffer for superresolution microscopy?

Dear researchers We are preparing for superresolution microscopy. I read some papers where the cells are prepared with the use of a special cytoskeletal buffer (CB) and followed by NaBH4...

20 February 2019 3,881 4 View

Is it possible to see a statistic regarding the amount of publications in year x with topic y?

Hallo, in the context of my master thesis I would like to point out that the publications in the area of the topic blockchain in supplychain management have increased enormously in the last two...

19 August 2018 9,225 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View