I have worked with U87MG and U251MG glioma cell lines and trypsinized both the cell lines for 30-40 seconds in 0.05% of trypsin/EDTA soln. Recently I have started doing primary culture from glioma tissues got from OT. During trypsinzation half of the cells trypsinised within 2-5 minute. But remaining half of the cells even after 5 minutes not trypsinised but its shape changed to round.
Ajay Kumar
Sometimes you don't get deadherence even with 0.25% trypsin dear Palaniswamy, as I use 0.25% trypsin-EDTA only and its really tough for even trpsin-EDTA to get complete deadherence within 5 minutes sometimes.. You can try continous shaking to remove the cells at 37 degree and then a gentle pipetting at the end....Hope it helps..
1 votes
1 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line
More Palaniswamy Ramaswamy's questions See All
Similar questions and discussions