How to calculate At%, Wt% and mol% of Dopants in Nanoparticle Synthesis in any methods?

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How to remove the salt and get purified DNA in Silica Magnetic bead-based Nucleic acid extraction?

I am doing nucleic acid extraction (manual silica magnetic bead-based) from the serum sample. After the extraction, I have taken the OD 260/280 in UV spec. There is no protein contamination. But...

04 November 2020 1,061 2 View

Could you tell me the composition of the wash buffer in RNA extraction (Silica Magnetic Bead-based) and its proportions?

I am doing silica magnetic beads-based RNA isolation. I am facing some impurity issues because of washing. So kindly share some best washing buffer composition of wash buffer for especially for...

01 November 2020 6,536 2 View

Can I use Phosphate Buffer for Antibody and Fluorescence conjugation?

I am going to use Phosphate Buffer (pH 6.5) is an alternative of MES buffer (pH 6.5) for Antibody and Fluorescent Microspheres conjugation procedure. The literature I am referring to mentions MES...

22 December 2019 794 3 View

Do I need to do a polymerization reaction under a nitrogen atmosphere?

I am polymerizing styrene with Potassium Peroxodi Sulfate and Acrylic acid by an emulsion polymerization method. The literature I am referring to mentions stirring the reagents with a nitrogen...

18 December 2019 8,158 4 View

For microRNA profiling which method is better RT-qPCR or NGS?

We are planning to do a miRNA profiling to distinguish between two disease conditions with similar and closely associated clinical conditions. Which method of profiling is suitable such as qPCR...

26 September 2017 5,471 7 View

What is the best method to identify the molecular target of an antibacterial compound isolated from natural product?

Transcriptomics have given some clue about the target, but not sure about the methods to confirm the target.

25 January 2016 6,900 4 View

Does anyone know how to use the Rotor Gene 6000 to measure telomere length?

I was wondering if there was any lab currently using the Rotor Gene 6000 machine to measure telomere length as there is no one in my lab that knows how to run a RT PCR?

15 January 2015 6,127 2 View

Is it ok to use a pooled sample as reference sample in real-time PCR?

I am working on U87 & U251 cell lines. After treatment in triplicates isolated total RNA to cDNA. While doing real-time PCR can I use the pooled cDNA from triplicates as reference sample for...

20 December 2014 2,201 6 View

Do you have any suggestions for complete trypsinisation of primary glioma culture?

I have worked with U87MG and U251MG glioma cell lines and trypsinized both the cell lines for 30-40 seconds in 0.05% of trypsin/EDTA soln. Recently I have started doing primary culture from glioma...

16 December 2014 5,341 3 View

What is the lowest concentration of the protein one could use in the CD measurements, so that the total absorbance is <1 on 10mm path length Cuvette?

I wish to measure the CD experiments on a peptide to test the effect of Potassium Phosphate buffer (10mM). The CD region that I am interested in the range of 180-200nm.

27 November 2014 8,600 10 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How can I measure the thickness of thin layers deposited on textured glass susbtrates?

I want to measure the thickness of aluminum oxide and titanium oxide layers deposited on textured glass substates. The layer thicknesses are typically below 100nm.

21 February 2021 5,465 4 View

How to do a surface treatment of uncoated TiO2 with polymer?

Basically, I would like to know how to treat or coat on the uncoated bare TiO2 (Ti-OH rich) surface with polymer which has anchoring groups in the polymer structure like trimethoxygroups. What is...

21 February 2021 4,657 6 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Gaurav Gupta

Hii,

You can estimate the amount of doping via four methods. 1. Go for EDS mapping 2. ICPMS 3. XRF 4. Rietveld refinement of X-ray data. Through occupancies yiou can get your values in at%. However, in refinement intially you have to chose the theoretical value of doping concentrations. After refinement you will get the values. EDS-mapping sometimes gives lesser concentration and so don't believe on EDS results too much. Better will be go for ICPMS and Rietveld refinement.

You can have a look at my papers. I have calculated through Refinement of X-ray data.