How to calculate At%, Wt% and mol% of Dopants in Nanoparticle Synthesis in any methods?

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How to remove the salt and get purified DNA in Silica Magnetic bead-based Nucleic acid extraction?

I am doing nucleic acid extraction (manual silica magnetic bead-based) from the serum sample. After the extraction, I have taken the OD 260/280 in UV spec. There is no protein contamination. But...

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Could you tell me the composition of the wash buffer in RNA extraction (Silica Magnetic Bead-based) and its proportions?

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Can I use Phosphate Buffer for Antibody and Fluorescence conjugation?

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Do I need to do a polymerization reaction under a nitrogen atmosphere?

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18 December 2019 8,158 4 View

For microRNA profiling which method is better RT-qPCR or NGS?

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26 September 2017 5,471 7 View

What is the best method to identify the molecular target of an antibacterial compound isolated from natural product?

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25 January 2016 6,900 4 View

Does anyone know how to use the Rotor Gene 6000 to measure telomere length?

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15 January 2015 6,127 2 View

Is it ok to use a pooled sample as reference sample in real-time PCR?

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Do you have any suggestions for complete trypsinisation of primary glioma culture?

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What is the lowest concentration of the protein one could use in the CD measurements, so that the total absorbance is <1 on 10mm path length Cuvette?

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Ester cleavage conditions?

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How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

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CGA 300 Regulator for CO2 cylinder?

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How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

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Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

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Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

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How can I measure the thickness of thin layers deposited on textured glass susbtrates?

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Any Homemade Organic Synthesis Practices?

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What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

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Jyoti Mayekar

Hello Vasantha

You will have to do XPS of your sample for that.

Vasantha Kumar Palaniswamy

Thank You, Prof. Jyoti Mayekar

Gaurav Gupta

Hii,

You can estimate the amount of doping via four methods. 1. Go for EDS mapping 2. ICPMS 3. XRF 4. Rietveld refinement of X-ray data. Through occupancies yiou can get your values in at%. However, in refinement intially you have to chose the theoretical value of doping concentrations. After refinement you will get the values. EDS-mapping sometimes gives lesser concentration and so don't believe on EDS results too much. Better will be go for ICPMS and Rietveld refinement.

You can have a look at my papers. I have calculated through Refinement of X-ray data.

Gaurav Gupta Thank You Sir.