I need to separate the extracellular matrix (mesoglea) of marine polyps (Aurelia auritas' scyphistoma and Clava multicornis).
If somebody has worked with small marine organisms, please share your protocol/procedure, please!
I have just published a paper about isolating ECM from human colon (you can download it from my Researchgate page). Have a look at the paper, you might have few hints.
Hello, I have a somewhat weird question: has anyone had problems with campylobacter growing in the second half of the strip GLU through ERO? Have you managed to correct it? There is some...
19 July 2024 3,094 2 View
which publication do you recommend for submitting an article on the philosophical and sociological analysis of AI vs Society
30 January 2024 3,918 2 View
preprints on various scientific topics
04 July 2023 5,965 3 View
Hi, I am looking for a company operating in the field of artificial meat. By operating, I mean production or advanced stage of pre-production. I am looking for because we have some good solutions...
24 November 2022 7,258 8 View
I have seen in the literature that short chain poly-lactic acid (polylactide; PLA) oligomers are water soluble. But I am unable to find a commercial source of this type of oligomers. Does anyone...
31 October 2022 1,419 6 View
Can anyone suggest a mercury-free LED light source for fluorescence microscopy? I'm looking for the simplest and most economical replacement for mercury bulbs. Not the complicated stuff with many...
05 October 2022 6,057 3 View
Please only answer if you have done this and do not send me paper links. Thanks. Hi technical Gurus :) So, I am working on a cytotoxicity assay based on the Roche's WST-1 reagent. Every setup is...
15 September 2022 1,611 0 View
For various reasons, I need to avoid DMSO with my cells. So I wonder if isopropyl alcohol (2-propanol) could be used instead of DMSO to create my stock solutions library? I have learned that the...
04 July 2022 3,655 1 View
Based on the literature analysis it could be concluded that one of the most common composition of synthetic lipids to prepare liposome mixture or model membrane (particularly in the work with...
28 June 2022 9,817 3 View
Hello to all I observed a strange phenomenon with CHO-K1 following the change of serum batch (it is surely the toxicity of this batch but I am very curious about the possible nature of this...
21 April 2022 6,157 3 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...
08 August 2024 6,690 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...
03 August 2024 2,366 0 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
We assume this to be true. Science leaves the era of mathematics and enters the era of matrix mechanics and the turning point is the discovery of numerical statistical theory called Cairo...
31 July 2024 3,900 2 View
What can you look for to know that the circuit model being used is overfitting the data. How do you know when overfitting is occurring?
31 July 2024 7,425 0 View
I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...
30 July 2024 969 0 View