I am culturing liver cancer cell line in serum free tumour sphere medium (DMEM/F12 + EGF + bFGF) and culturing them for at least 7 days to enrich for cancer stem cells. Do I need to change the medium everyday?
I have a concern that this will disturb the sphere formation as I will need to centrifuge and I am afraid that during the resuspension in fresh media will affect the spheres. I am culturing them in 6-well ultra low plate by the way.
Saimonas Raudonius
Hello,
You really don't need to change the medium every day, but it depends on the amount of spheres that are current in the well. We've cultured cancer stem cell spheroids in Petri dishes, the centrifugation did not affect their stability or behaviour. And the addition of EGF and bFGF is unnecessary.
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