8 Questions 5 Answers 0 Followers
Questions related from Ain Zubaidah Ayob
Hi everyone.. is it possible to analyse our data saved as JPEG in ImageJ? I did tumourspheres culture under hypoxic condition and have to take the pictures of my spheres in multiple 6 well plates,...
26 March 2018 6,883 2 View
I am using Sigma DNase I (AMPD1) where I add 1 ul of each component(DNAse1, buffer and stop solution) to 8 ul of RNA in water (below is the link to the...
22 November 2016 3,327 2 View
I am having trouble getting enough protein to run SDS-PAGE, I barely get 50-60 ug total protein as my total concentration in a final volume of 150 ul. I grow my tumoursphere in Corning ULA 6 well...
16 February 2016 8,327 3 View
I am culturing liver cancer cell line in serum free tumour sphere medium (DMEM/F12 + EGF + bFGF) and culturing them for at least 7 days to enrich for cancer stem cells. Do I need to change the...
04 November 2015 4,166 1 View
I know from ATCC/JCRB depository saying that these cells are different based on their source and the presence of HBV/HCV. What kind of other consideration when I'm looking into working with more...
27 September 2015 2,349 6 View
Anyone mind to share their HepG2 cells morphology here? I am now getting different cell morphology (most probably due to my technique) and I keep getting clumpy cells and the borders between the...
10 September 2015 8,010 10 View
Hi, I am trying to generate a spheroid to culture HepG2 cells , and my objective is to evaluate the effect of different culture conditions on the marker expression. Not really on the optimisation...
02 August 2015 9,490 17 View
I am planning to do western blot for a start to analyse CD133 expressions in my cells, and therefore are using unconjugated antibody, which I hoped can be used for other applications such as...
06 February 2015 6,427 2 View
