12 Questions 6 Answers 0 Followers
Questions related from Ain Zubaidah Ayob
I have performed a matched sample miRNA and mRNA profiling to identify the potential mechanisms that regulate aspects that are associated with cancer stem cell properties in my treated samples. I...
26 April 2020 9,450 0 View
Hi everyone.. is it possible to analyse our data saved as JPEG in ImageJ? I did tumourspheres culture under hypoxic condition and have to take the pictures of my spheres in multiple 6 well plates,...
26 March 2018 6,901 2 View
I am using Sigma DNase I (AMPD1) where I add 1 ul of each component(DNAse1, buffer and stop solution) to 8 ul of RNA in water (below is the link to the...
22 November 2016 3,336 2 View
I am having trouble getting enough protein to run SDS-PAGE, I barely get 50-60 ug total protein as my total concentration in a final volume of 150 ul. I grow my tumoursphere in Corning ULA 6 well...
16 February 2016 8,339 3 View
I am culturing liver cancer cell line in serum free tumour sphere medium (DMEM/F12 + EGF + bFGF) and culturing them for at least 7 days to enrich for cancer stem cells. Do I need to change the...
04 November 2015 4,178 1 View
I am trying to transiently induce EMT in HepG2 cells using TGFb/Activin recombinant proteins. What should be the ideal confluency before starting the treatment? And is serum starvation required...
02 October 2015 7,746 1 View
I know from ATCC/JCRB depository saying that these cells are different based on their source and the presence of HBV/HCV. What kind of other consideration when I'm looking into working with more...
27 September 2015 2,368 6 View
Anyone knows any good company selling hepatic liver stem cells?
14 September 2015 4,726 4 View
Anyone mind to share their HepG2 cells morphology here? I am now getting different cell morphology (most probably due to my technique) and I keep getting clumpy cells and the borders between the...
10 September 2015 8,019 10 View
Hi, I am trying to generate a spheroid to culture HepG2 cells , and my objective is to evaluate the effect of different culture conditions on the marker expression. Not really on the optimisation...
02 August 2015 9,503 17 View
I am planning to do western blot for a start to analyse CD133 expressions in my cells, and therefore are using unconjugated antibody, which I hoped can be used for other applications such as...
06 February 2015 6,442 2 View
I am trying to stain my marker that are both localised on the membrane and in the cytoplasm. I am not sure on how to go about the antibody staining - should I do it one incubation at a time or is...
08 January 2015 9,051 4 View
