Is melatonin tablets given for sleep disorders likely to induce a negative feedback effect on the endogenous secretion of melatonin?

Hi! Melatonine is indicated in Europe for sleep disorders in patients > 65 years old . The secretion of melatonin is regulated by light. Apparently, the following mechanism is observed: absence...

09 October 2018 2,099 3 View

Why the hypnotic effect of the anti histaminic drugs is descreased after few days?

Anti-histaminergic (H1 antagonists) drugs could be prescribed at low doses for their sedative effects. Nevertheless, some reviews and articles reported a decrease of sedative effect that can occur...

05 June 2018 6,583 7 View

Which protolcol to properly perform immunoprecipitation anti-V5 and mass spect analyses?

10 November 2016 4,792 2 View

Are SH-SY5Y cells the best model to study toxicity on dopaminergic cells ?

07 August 2016 4,244 6 View

Do contaminating proteins affect the infectivity of AAVs?

04 May 2016 3,801 3 View

What is the best serotype of AAV for transducing neuronal and atroglial cells primary culture of rat/mice ?

03 April 2016 5,296 8 View

We co-transfected HEK cells with plasmid containing CMV and NRE : Is it possible to have a competition between the two promoters?

Hello, I am looking for article references that evaluate interactions between two promoters in a context of co-transfection in HEK cells: It seems that we have interactions : the plasmid...

11 December 2015 5,114 3 View

How can I improve the penetration of DNA into the muscle?

09 October 2015 1,777 3 View

Are mice C57Bl6J from the strain RccHSD from Harlan laboratories suitable for a model of Parkinson's disease?

03 April 2015 2,620 6 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View