Hello, I am looking for article references that evaluate interactions between two promoters in a context of co-transfection in HEK cells:
It seems that we have interactions : the plasmid containing the CMV decreased the activity of the NRE : Is it possible?
Hello Marine,
Yes, it is. I have experienced such a competition while cotransfecting my cells using a target plasmid and a control plasmid. To confirm that you can carry out a dual luciferase assay if your plasmids are constructed for that.
Hope it helps.
Best,
Hamadi.
Yes, it helps, thank you ! .Would you know any published datas about this interactions ?
That's great Marie! Unfortunetly, I couldn't send you my data since I am not allowed to do that as an internal rule. Soryy for that :(
Best,
Hamadi
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