Is melatonin tablets given for sleep disorders likely to induce a negative feedback effect on the endogenous secretion of melatonin?

Hi! Melatonine is indicated in Europe for sleep disorders in patients > 65 years old . The secretion of melatonin is regulated by light. Apparently, the following mechanism is observed: absence...

09 October 2018 2,099 3 View

Why the hypnotic effect of the anti histaminic drugs is descreased after few days?

Anti-histaminergic (H1 antagonists) drugs could be prescribed at low doses for their sedative effects. Nevertheless, some reviews and articles reported a decrease of sedative effect that can occur...

05 June 2018 6,583 7 View

Do 9L cells express GDNF receptors ?

10 November 2016 9,604 1 View

Are SH-SY5Y cells the best model to study toxicity on dopaminergic cells ?

07 August 2016 4,244 6 View

Do contaminating proteins affect the infectivity of AAVs?

04 May 2016 3,801 3 View

What is the best serotype of AAV for transducing neuronal and atroglial cells primary culture of rat/mice ?

03 April 2016 5,296 8 View

We co-transfected HEK cells with plasmid containing CMV and NRE : Is it possible to have a competition between the two promoters?

Hello, I am looking for article references that evaluate interactions between two promoters in a context of co-transfection in HEK cells: It seems that we have interactions : the plasmid...

11 December 2015 5,114 3 View

How can I improve the penetration of DNA into the muscle?

09 October 2015 1,777 3 View

Are mice C57Bl6J from the strain RccHSD from Harlan laboratories suitable for a model of Parkinson's disease?

03 April 2015 2,620 6 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View