Marie Humbert-Claude

10 Questions 14 Answers 0 Followers

Questions related from Marie Humbert-Claude

Is melatonin tablets given for sleep disorders likely to induce a negative feedback effect on the endogenous secretion of melatonin?

Hi! Melatonine is indicated in Europe for sleep disorders in patients > 65 years old . The secretion of melatonin is regulated by light. Apparently, the following mechanism is observed: absence...

10 October 2018 2,128 3 View

Why the hypnotic effect of the anti histaminic drugs is descreased after few days?

Anti-histaminergic (H1 antagonists) drugs could be prescribed at low doses for their sedative effects. Nevertheless, some reviews and articles reported a decrease of sedative effect that can occur...

06 June 2018 6,605 7 View

Do 9L cells express GDNF receptors ?

Hello, GDNF exposure seems to induce proliferation of 9L cells in culture. Do somebody know if 9 L Cell line ( gliosarcoma from rats) express the GFRalpha1/RET recptors?

11 November 2016 9,626 1 View

Which protolcol to properly perform immunoprecipitation anti-V5 and mass spect analyses?

I would like to perform immunoprecipitation with an anti-v5 antibody followed by a mass spect analyses, on brain extract tissue. Where may I find the most suitable protocol ? ( anti-v5...

11 November 2016 4,818 2 View

Are SH-SY5Y cells the best model to study toxicity on dopaminergic cells ?

Hello ! I am searching an in vitro model to study the implication of a protein in mitochondrial dysfunctions in dopaminergic cells. Are SH-SY5Y the best model ? Are primary culture cells from...

08 August 2016 4,266 6 View

Do contaminating proteins affect the infectivity of AAVs?

Hello! I currently observe discrepancies between qPCR titers and in vivo efficiency of our AAV2/1 and AAV2/5 injected into the brain. My qPCR quality controls are good (no contamination,...

05 May 2016 3,829 3 View

What is the best serotype of AAV for transducing neuronal and atroglial cells primary culture of rat/mice ?

I would like to transduce neuronal and astrogilal primary culture cells from mice or rats with an AAV. Do you know a reliable article that allows to answer to these questions : Should I produce...

04 April 2016 5,329 8 View

We co-transfected HEK cells with plasmid containing CMV and NRE : Is it possible to have a competition between the two promoters?

Hello, I am looking for article references that evaluate interactions between two promoters in a context of co-transfection in HEK cells: It seems that we have interactions : the plasmid...

12 December 2015 5,139 3 View

How can I improve the penetration of DNA into the muscle?

We want to analyze the immune response against a protein. Our approach is to make intra-muscular injections of a plasmid that encodes for the expression of the protein. (We do not have enough AAV...

10 October 2015 1,829 3 View

Are mice C57Bl6J from the strain RccHSD from Harlan laboratories suitable for a model of Parkinson's disease?

The mice from the strain C57Bl6J OLA HSD from Harlan Laboratories are mutated on the alphasynuclein gene. We have spent lot of time understanding why, after injection of LPS into the nigra, the...

04 April 2015 2,646 6 View