We want to analyze the immune response against a protein. Our approach is to make intra-muscular injections of a plasmid that encodes for the expression of the protein. (We do not have enough AAV to do this experiment and we do not have electric devices for proceeding to an additional electric choc after injection.)
Dear Marie,
you could add an adjuvant to the DNA preparation. Cardiotoxin is frequently used for improving DNA vaccines.
Greetings
Christoph
U also can try administration with modified probiotics as experimental adjuvants. Thanks
You can use co-polymers ( see ref: Chèvre R, Le Bihan O, Beilvert F, Chatin B, Barteau B, Mével M, et al.
Amphiphilic block copolymers enhance the cellular uptake of DNA molecules
through a facilitated plasma membrane transport. Nucleic Acids Res 2011;39:
1610–22.
Beilvert F, Tissot A, Langelot M, Mével M, Chatin B, Lair D, et al. DNA/amphiphilic
block copolymer nanospheres reduce asthmatic response in a mouse model of
allergic asthma. Hum Gene Ther 2012;23:597–608.)
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