Random seed number and number of simulation run?

hello everyone After I calibrated my average delay at roundabout using simulation random seed number of 42, then I tried to run the model 5 runs using different seed numbers, but I get different...

19 June 2019 3,972 3 View

Why different coating of a sample for scanning electron microscopy gave a different result?

We did Scanning Electron Microscopy and found that our result is not satisfactory. We used two types of coating. Initially, it was chromium coating. However, the focus of the SEM to our samples...

10 April 2018 1,981 20 View

What is the temperature range that may break a chemical bond; single/double/ etc.?

08 May 2017 9,857 4 View

Does anyone know which membrane is good for bioethanol purification?

02 March 2015 4,976 2 View

Does anyone have knowledge of acid leaching gibbsitic bauxite with HCl to extract Al and Fe?

The best condition of leaching process, AlCl3.6H2O precipitation and hydrothermal process to get ferric oxide

01 February 2014 6,078 2 View

What influence the lowering onset temperature of starch?

We are doing a variety of treatments to starch in order to modify the properties. However, one of the samples shows a lowering of onset temperature via DSC compared to the control treatment. One...

01 January 1970 3,128 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View