Hi,
I want to prepare 100 uM Coniferyl Alcohol solution to use for enzymatic assay reaction.
Here is the detail of the product
1. Synonym: 3-(4-Hydroxy-3-methoxyphenyl)-2-propen-1-ol, 4-Hydroxy-3-methoxycinnamyl alcohol
2. Brand: Sigma Aldrich
3. Molecular formula: HOC6H3(OCH3)CH=CHCH2OH
4. MW: 180.20 g/mol
May I know what reagent that I need to use to dissolve the chemical? Also, after I prepared the solution, do I need to kept the solution in dark and cold condition?
Your answer is much appreciated
A commonly used water-miscible solvent for compounds that are to be tested in enzyme assays, if they won't dissolve in the assay buffer, is dimethylsulfoxide (DMSO). Enzymes can usually tolerate a few % DMSO by volume, but you should keep a constant percentage of DMSO in all samples in case your enzyme's activity is affected.
Since this compound is probably unstable in air due to the double bond, I suggest you make a fresh solution immediately before running the assay.
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