Yr Hwang

3 Questions 1 Answers 0 Followers

Questions related from Yr Hwang

CRISPR activation in a cell type normally not expressing target gene?

hi I tried working CRISPR activation system to overexpress a gene which is normally not expressed in the target cell. but I failed it. I delivered each plasmids using aav (2wks infect) and already...

04 July 2024 3,085 0 View

Different results from western blot and icc?

hi have you ever experienced of coming different results from western blot and icc? I treated toxin (a-syn PFF) to control ES-derived midbrain doparminergic neuron and experimental group that I...

26 May 2024 3,205 0 View

How to validate sgRNA for cripsra?

hi i'm gonna use crispra system for my study, how can i validate sgRNA not having off-target effect?

14 October 2023 6,767 0 View