13 September 2023 0 1K Report

I found two papers to establish a Thp-1 differentiation protocol (Thp-1 -(PMA)-> M0 -(LPS+IFN or IL-4+IL-13)->M1 or M2). I want to compare both protocols, but the PMA priming times and the types of confirmed M1 and M2 markers are completely different. I'm not sure which one to choose. I need your opinion. Or if there's a better protocol other than these two, please let me know.

1) Genin, M., Clement, F., Fattaccioli, A. et al. M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide. BMC Cancer 15, 577 (2015). https://doi.org/10.1186/s12885-015-1546-9

2) Baxter, E.W., et al., Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFNγ+LPS), M(IL-4) and M(IL-10) phenotypes. Journal of Immunological Methods, 2020. 478: p. 112721.

Thank you in advance!

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