Can you explain me about the principle of Spatial transcriptomics?

25 December 2023 1 6K Report

I'm trying to study spatial transcriptomics by myself, but it's not easy to understand. So, spatial GEX is to cut the tissue into slides, attach it to the slide where the probes are located, and then produce a spatially coded cDNA... What does this mean? And what is UMI? If you don't mind, please explain the figure below.

Intae Hwang

Oh I figured it out by myself!

Badges
Science topic

More Intae Hwang's questions See All

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning climate change?

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning time-dependent temperature, relative humidity and concentration of carbon dioxide?

08 February 2021 4,277 1 View

Habitat Suitability through GIS without presence-absence data?

What kind of models/techniques can be used with GIS spatial analysis in order to determine habitat suitability for a species that is not (widely) present in a certain region? For my bachelor...

31 January 2021 2,777 2 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View

How can I implement a Generalized Estimating Equation method on spatially autocorrelated time-series data?

I used Generalized Estimating Equations (geepack in R) on my regularly monitored (twice a year) plant abundance data in grid cells to evaluate their trends of density. Since these are time-series...

26 January 2021 3,833 1 View

What is your raw statistical treatment preference?

Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...

22 January 2021 1,527 3 View

How to plot the significance level using line or dot pattern over a raster data plot in R usign base plot function?

After plotting the raster data using the plot() function in 'R'. How can we plot the other layer overlayed with dot/line pattern in place of color fill as shown in the picture...

06 January 2021 5,865 4 View

Do you think that GIS is going to vanish in next ten years or visa versa?

Smart application and Google earth and google maps are now becoming a huge geo spatial data base and this may decrease the use of software related to GIS

29 December 2020 3,940 12 View

How do I plot the correlation coefficients (r) on a map based on a list of variables?

I am working to improve a manuscript and I have been advised to provide a map showing where the correlation is significant. Any information or links to learn about this would be quite helpful....

26 December 2020 1,162 5 View

Is (Are) there any valid corpus containing geographical events queries?

Hi all, I'm currently looking for a question corpus to have a plausible number of spatial events queries. I found MS MARCO dataset in which I could extract a reasonable number of geographical...

08 December 2020 7,844 2 View