When I culture Candida albicans biofilm in 48-wells plate for one day, some floating pieces could be observed in the liquid-air interface. Is it the broken biofilm?
My biofilm culture procedure: After initial adhesion for 90 min, unattached Candida albicans cells were removed and the 48-wells plate was washed by PBS. After PBS removal, the culture media is added. And the plate is settled in incubator with shaking rate of 180 rpm.
Try incubating without shaking and look if you find floating circular biofilms or ones growing from the wall of the plate.
Candida albicans shown to produce biofilms on a variety of surfaces, including liquid-gas and liquid-solid interfaces. Biofilm creation is a complicated process that begins with microbial cell adhesion to a surface and ends with the synthesis of an extracellular matrix that encases the cells and gives structural support to the biofilm community.
Biofilm development by Candida albicans can occur at both liquid-gas and liquid-solid interfaces. Candida albicans biofilms, for example, can grow on medical equipment such as catheters and implants in the setting of medical infections, implying a liquid-solid interface. Candida biofilms, on the other hand, have been examined in laboratory settings where they may grow at the air-liquid interface in liquid culture systems. Peng Dong
Dear peng dong
Only the above mentioned procedures you did in your biofilm production assay Peng Dong
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