Hi, i ordered DAB from sigma:
https://www.sigmaaldrich.com/catalog/product/sial/d8001?lang=en®ion=CA
and I tried dissolving it in water, MES, and even pH lowered to 3 it still wont dissolve and stain my samples. Doe anyone have this problem??
What was the % of DAB you try to dissolved?
We following the following protocol to prepare 1% DAB: Add 0.1g DAB in 10 ml of distilled water and add 3 to 5 drops of 10 N Hcl to lower the pH. Vortex solution until DAB completely dissolves, it takes about 10 minutes. The solution turn to light brown color. The solubility of DAB generally requires an acidic pH; lower the pH if DAB does not dissolve.
Hope it helps
In addition to the helpful suggestions above you can also heat the buffer up to 50 degrees C and do the resuspension according to Manoj. The warmth helps to resuspend the DAB faster.
Cheers, Christian
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