Malaika Gomes

5 Questions 3 Answers 0 Followers

Questions related from Malaika Gomes

At what concentration does hydrogen peroxide inhibit enzyme activity?

I am trying to measure GUS expression, and was wondering if anyone knew what concentration hydrogen peroxide inhibits the GUS protein??? Thanks ! Any help is appreciated!

24 November 2020 4,215 3 View

What else reduces NBT (nitroblue tetrazolium) to formazan?

I need to produce formazan to make a standard curve, (as in this paper:Quantitative Determination of Superoxide in Plant Leaves Usi... ) The reaction requires riboflavin and methionine,...

06 March 2020 6,556 3 View

DAB (3'3 diaminobenzidine) will not dissolve?

Hi, i ordered DAB from sigma: https://www.sigmaaldrich.com/catalog/product/sial/d8001?lang=en®ion=CA and I tried dissolving it in water, MES, and even pH lowered to 3 it still wont...

31 January 2020 3,933 3 View

CRISPR efficiency based on amount of off-target sites?

Hi, When designing my CRISPR guide RNAs, to knockout regions of my gene of interest's promoter, I used the CRISPR-P software (http://crispr.hzau.edu.cn/cgi-bin/CRISPR2/CRISPR). However, where I...

25 July 2019 3,411 0 View

For CRISPR, does it matter if the guide RNA is looking for the antisense or sense strand?

So, I've used the CRISPR P 2.0 software to look up guide RNAs to use to delete a region in my gene's promoter. Past members of our lab were using one guide RNA taken straight from that website to...

02 November 2018 1,861 2 View