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Questions related from Malaika Gomes
I am trying to measure GUS expression, and was wondering if anyone knew what concentration hydrogen peroxide inhibits the GUS protein??? Thanks ! Any help is appreciated!
24 November 2020 4,211 3 View
I need to produce formazan to make a standard curve, (as in this paper:Quantitative Determination of Superoxide in Plant Leaves Usi... ) The reaction requires riboflavin and methionine,...
06 March 2020 6,555 3 View
Hi, i ordered DAB from sigma: https://www.sigmaaldrich.com/catalog/product/sial/d8001?lang=en®ion=CA and I tried dissolving it in water, MES, and even pH lowered to 3 it still wont...
31 January 2020 3,931 3 View
Hi, When designing my CRISPR guide RNAs, to knockout regions of my gene of interest's promoter, I used the CRISPR-P software (http://crispr.hzau.edu.cn/cgi-bin/CRISPR2/CRISPR). However, where I...
25 July 2019 3,409 0 View
So, I've used the CRISPR P 2.0 software to look up guide RNAs to use to delete a region in my gene's promoter. Past members of our lab were using one guide RNA taken straight from that website to...
02 November 2018 1,861 2 View