We already used milk: 5% to 1 hour, 7% to 2 hour, 10% to 1h, 5% overnight, but, , in all blockages it still marks the entire membrane, every run, there is a large band with no specific mark
HELP
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,366 3 View
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
What is the future scope of acoustic emission?
17 July 2024 1,510 1 View
I have a small molecule reagent with a boc-protected amino group. Now the reaction needs to be reacted at 37°C for 30 h. Is this protection group stable?
12 July 2024 3,745 2 View
I am trying to measure the plasmon resonance angle of gold film and pure water using the Kretschmann configuration and a 633nm laser. Without flowing water over the gold, I can detect a clear...
10 July 2024 4,719 3 View
Hello! I am facing a problem, I tried using pdb2gmx to generate topology file but it shows error HEA residue not found then I tried copying the forcefield file and edited accordingly but again...
24 June 2024 1,242 0 View
I'm conducting research on photoplethysmography (PPG) signals obtained from smartwatches worn on the wrist. The main goal is to analyze the PPG waveform and extract key fiducial points and...
13 June 2024 3,286 0 View
The Acid aging in paper samples
10 June 2024 6,456 2 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,293 3 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...
29 July 2024 9,572 3 View
Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...
28 July 2024 6,553 1 View