What is the best way to quantify astrocytes and microglia in tissue?

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Louis-Charles Béland

I always use fluorescence intensity (using Iba1 or CD11b as markers). Sometimes, upon activation, microglia can get syncitial which makes cell counting difficult.

Ami Mehta

Quantifying either through immunofluorescence or flow cytometry is the best option. Try labelling the astrocytes and microglia with markers such as GPAP (for astrocytes) and Pro-Fcgr3 (for microglia). Cell counting may also be too confusing due to similar morphologies.

Samir Ranjan Panda

Hi Beatriz Botasso Gomes

To confirm microglia activation and phagocytosis (IBA-1+CD-68) and astrocyte activation (GFAP). Measuring fluorescence intensity is mostly used for the analysis.

Thanks,