How to troubleshoot the IF staining problems in skin tissues?

Hi We are working in the area of psoriatic diseases. We are everytime getting the problem in expression of protein in dermis. We unable to see the expression in epidermis. Even, we did IHC too...

10 November 2018 9,096 1 View

How to process the mouse skin tissues for histopathological evaluation?

Hi everybody, In our studies, we have used BALB/c mice for antipsoriatic activity evaluation. In that, we unable to get the proper tissue sections for histophathological and immunofluorescence...

05 June 2018 798 3 View

What are the biological assays for drug and TNF Alfa binding studies?

Dear sir/madam, Please suggest the biological assays which need to prove the drug and TNF Alfa binding studies. Thanks.

05 June 2018 5,334 1 View

How can I conjugate the miRNA with cy5 dye for tracking the transfection?

Hi everybody, I would like to transfect the anti-miR to the cancer cells. I have purchased it from sigma. I have the lipofectamine 2000 as a transfection agent.But I don't know the procedure to...

05 June 2016 1,110 2 View

How can I give the anti-miRNA to the athymic nude mice?

Hi everybody, I want to give the anti-miR as a therapeutic agent for the tumor bearing mouse. I have purchased the anti-miR from sigma Aldrich. Is there any standard protocol to deliver the...

05 June 2016 8,527 2 View

For getting Post-Doc in top universities, is there any requirement of guide profile and institution name?

10 November 2015 1,007 2 View

What are the requirements to maintain the cancer stem cells?

We have normal in vitro facilities to culture the normal cancer cells. I want to work on cancer stem cells. Could you please suggest me what are the effective methods to maintain the cancer stem...

09 October 2015 5,371 5 View

Could you please tell me the methods to isolate cancer stem cells without the application of FACS?

I want to isolate the cancer stem cells from cancer cell lines. But most of the people are isolating the cancer stem cells by using FACS. But in my lab it is not available. So I want to use...

09 October 2015 8,478 5 View

Why do I amplification in RT-PCR in RNA-free water?

I am using Taqman miRNA assay kit for miRNA amplification. But I am getting the peak for water control also. I have repeated by using new purchased RNAse free water, even-though I am getting same...

04 May 2015 5,874 7 View

How can I detect the mature miRNA?

I am going to detect miR-146a in human cell lines by RT PCR. I found most of the people are using the taqman assay kit. In my lab superscript III SYBR green method is already established and we...

11 December 2014 7,446 9 View

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02 March 2021 3,060 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View