13 Questions 24 Answers 0 Followers
Questions related from Pooladanda Venkatesh
Hi We are working in the area of psoriatic diseases. We are everytime getting the problem in expression of protein in dermis. We unable to see the expression in epidermis. Even, we did IHC too...
11 November 2018 9,157 1 View
Dear sir/madam, Please suggest the biological assays which need to prove the drug and TNF Alfa binding studies. Thanks.
06 June 2018 5,390 1 View
Hi everybody, In our studies, we have used BALB/c mice for antipsoriatic activity evaluation. In that, we unable to get the proper tissue sections for histophathological and immunofluorescence...
06 June 2018 854 3 View
I'm trying to disperse it in Distilled water, and used sonication and homogenization approach to reduce its size but still getting PDI more than 0.5 and size more than 600nm
08 August 2017 5,126 4 View
Hi everybody, I want to give the anti-miR as a therapeutic agent for the tumor bearing mouse. I have purchased the anti-miR from sigma Aldrich. Is there any standard protocol to deliver the...
06 June 2016 8,586 2 View
Hi everybody, I would like to transfect the anti-miR to the cancer cells. I have purchased it from sigma. I have the lipofectamine 2000 as a transfection agent.But I don't know the procedure to...
06 June 2016 1,171 2 View
If we have good background of scientific journals means we can able to get post-doc in good institutions, it may possible or not?
11 November 2015 1,081 2 View
We have normal in vitro facilities to culture the normal cancer cells. I want to work on cancer stem cells. Could you please suggest me what are the effective methods to maintain the cancer stem...
10 October 2015 5,452 5 View
I want to isolate the cancer stem cells from cancer cell lines. But most of the people are isolating the cancer stem cells by using FACS. But in my lab it is not available. So I want to use...
10 October 2015 8,568 5 View
I am using Taqman miRNA assay kit for miRNA amplification. But I am getting the peak for water control also. I have repeated by using new purchased RNAse free water, even-though I am getting same...
05 May 2015 5,931 7 View
Hi I am unable to get p-EGFR blots clearly as compare to Actin and others. Even tried with more concentration antibody also. I am running the gel which contains 7.5%. I am keeping 1.30 min for...
12 December 2014 7,208 1 View
I am going to detect miR-146a in human cell lines by RT PCR. I found most of the people are using the taqman assay kit. In my lab superscript III SYBR green method is already established and we...
12 December 2014 7,519 9 View
Hi Please reply me the proper solutions, I am frustrating with A549 cell lines. Whenever I have revived fresh vial from the liquid nitrogen I am not getting problem up to 3 splits after that the...
11 November 2014 7,792 14 View
