Pooladanda Venkatesh

13 Questions 24 Answers 0 Followers

Questions related from Pooladanda Venkatesh

How to troubleshoot the IF staining problems in skin tissues?

Hi We are working in the area of psoriatic diseases. We are everytime getting the problem in expression of protein in dermis. We unable to see the expression in epidermis. Even, we did IHC too...

11 November 2018 9,146 1 View

What are the biological assays for drug and TNF Alfa binding studies?

Dear sir/madam, Please suggest the biological assays which need to prove the drug and TNF Alfa binding studies. Thanks.

06 June 2018 5,383 1 View

How to process the mouse skin tissues for histopathological evaluation?

Hi everybody, In our studies, we have used BALB/c mice for antipsoriatic activity evaluation. In that, we unable to get the proper tissue sections for histophathological and immunofluorescence...

06 June 2018 844 3 View

How to disperse ZrO2 nanopowder in vehicle suitable for its use in cell culture.....

I'm trying to disperse it in Distilled water, and used sonication and homogenization approach to reduce its size but still getting PDI more than 0.5 and size more than 600nm

08 August 2017 5,117 4 View

How can I give the anti-miRNA to the athymic nude mice?

Hi everybody, I want to give the anti-miR as a therapeutic agent for the tumor bearing mouse. I have purchased the anti-miR from sigma Aldrich. Is there any standard protocol to deliver the...

06 June 2016 8,576 2 View

How can I conjugate the miRNA with cy5 dye for tracking the transfection?

Hi everybody, I would like to transfect the anti-miR to the cancer cells. I have purchased it from sigma. I have the lipofectamine 2000 as a transfection agent.But I don't know the procedure to...

06 June 2016 1,161 2 View

For getting Post-Doc in top universities, is there any requirement of guide profile and institution name?

If we have good background of scientific journals means we can able to get post-doc in good institutions, it may possible or not?

11 November 2015 1,069 2 View

What are the requirements to maintain the cancer stem cells?

We have normal in vitro facilities to culture the normal cancer cells. I want to work on cancer stem cells. Could you please suggest me what are the effective methods to maintain the cancer stem...

10 October 2015 5,437 5 View

Could you please tell me the methods to isolate cancer stem cells without the application of FACS?

I want to isolate the cancer stem cells from cancer cell lines. But most of the people are isolating the cancer stem cells by using FACS. But in my lab it is not available. So I want to use...

10 October 2015 8,554 5 View

Why do I amplification in RT-PCR in RNA-free water?

I am using Taqman miRNA assay kit for miRNA amplification. But I am getting the peak for water control also. I have repeated by using new purchased RNAse free water, even-though I am getting same...

05 May 2015 5,924 7 View

How to detect p-EGFR on western blot with low background?

Hi I am unable to get p-EGFR blots clearly as compare to Actin and others. Even tried with more concentration antibody also. I am running the gel which contains 7.5%. I am keeping 1.30 min for...

12 December 2014 7,195 1 View

How can I detect the mature miRNA?

I am going to detect miR-146a in human cell lines by RT PCR. I found most of the people are using the taqman assay kit. In my lab superscript III SYBR green method is already established and we...

12 December 2014 7,504 9 View

What is the solution for the following problem with A549 cell lines?

Hi Please reply me the proper solutions, I am frustrating with A549 cell lines. Whenever I have revived fresh vial from the liquid nitrogen I am not getting problem up to 3 splits after that the...

11 November 2014 7,786 14 View