I want to perform umbilical cord blood culture for cytogenetic studies, but the 72-hour cultures do not give me results.
Tanks.
What are the recommended fluorophores for in vivo tumor imaging using the IVIS system? I am particularly interested in options compatible with IVIS imaging and would like information on the limit...
19 November 2023 4,004 0 View
There are a lot of factors that can contribute in bacterial antibiotic resistance. What do you think is the most significant factor in bacterial antibiotic resistance?
23 February 2023 7,770 7 View
I have read many protocols for the preparation of cross-linked hyaluronic acid gel for soft tissue fillers, but cross-linking active ingredients are hard to find in my country, so I am looking for...
05 February 2023 3,961 0 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 5,466 3 View
From a population of transfected/transduced cells I sort those with the desired expression. Starting from 10.000 cells I extract the ARN, and from its cDNA I amplify the gene of interest, as well...
19 September 2020 8,523 3 View
I believe by using greater number of antennas in simulation, SNR would also be improved. However I have no proof for that. Are there any equations that support my idea?
25 September 2019 5,070 3 View
the DSC results for a blend containing 90% HDPE and 10% polyethylene wax shows two separate peaks during the first heating, one for the wax phase and one for the HDPE phase. but the peak...
08 May 2019 3,832 7 View
I want to design a horn antenna which only produces incident field along theta (it's got copolarization along theta). how can I do that?
05 February 2019 10,013 3 View
Hi! I use this protocol: Purification and Properties of an Extraeellular Endo-l,4-Glucanase from Lenzites trabea (Herr, et al 1978), but i would like to know if there are protocols standardized by...
13 August 2018 350 2 View
I am currently in the process of deciding on a research question (and an according method/research design) for my master thesis in the named field. So far, I have not been able to find an open...
06 August 2018 5,929 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View