Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Contributions to Nanomaterials Special Issue "Porous Metal-Organic Framework Nanoparticles"?

20 October 2020 248 2 View

Advices for: no amplification of aimed gene from transfected cells seleceted for it?

From a population of transfected/transduced cells I sort those with the desired expression. Starting from 10.000 cells I extract the ARN, and from its cDNA I amplify the gene of interest, as well...

19 September 2020 8,378 3 View

Does anybody know if there are antibodies targetting HIV TAT-peptide?

Does anybody know if there are antibodies targetting HIV TAT-peptide (just the region used for transduction: GRKKRRQRRRPQ) We wanted know if our TAT-peptide has entered the cell by Western Blott...

29 September 2019 7,692 1 View

The effect of number of antennas in multistatic architecture for imaging and SNR?

I believe by using greater number of antennas in simulation, SNR would also be improved. However I have no proof for that. Are there any equations that support my idea?

25 September 2019 4,890 3 View

Does anybody know if there are antibodies targetting HIV TAT-peptide?

Does anybody know if there are antibodies targetting HIV TAT-peptide (just the region used for transduction: GRKKRRQRRRPQ) We wanted know if our TAT-peptide has entered the cell by Western Blott...

08 September 2019 9,239 1 View

What is the general trend of I-V Characteristic graph for silicate based glasses?

What is the general trend of I-V Characteristic graph for silicate based glasses?

14 July 2019 1,481 4 View

Why does a DSC peak disappear during the second heating?

the DSC results for a blend containing 90% HDPE and 10% polyethylene wax shows two separate peaks during the first heating, one for the wax phase and one for the HDPE phase. but the peak...

08 May 2019 3,580 7 View

How can I define the polarization of my incident field in CST for my horn antenna?

I want to design a horn antenna which only produces incident field along theta (it's got copolarization along theta). how can I do that?

05 February 2019 9,911 3 View

Does anyone know of any dialysis membrane NOT made of organics (e.g. cellulose, etc.)?

I am performing some experiments and I'd need to buy a dialysis membrane (not a filtration membrane) that is not made of organic molecules. Teflon (C-F) would work, but haven't been able to find...

29 October 2018 2,906 2 View

Could anyone recommend papers that focus on mixed-ethnicity Black-Caribbean/Caucasian adopted children?

Dear All, I have been looking for papers, or books, that focus on the issues surrounding adoption of mixed Black-Caribbean/Caucasian children. I am interested in literature that looks at any...

22 February 2021 8,466 2 View

What/How to focus on with regard to research publication?

I'm an academician for past 10 years and love to continue with the same, a question pops-up at in mind from time to time, what to focus more on? Conference papers, Journal paper, book chapters,...

09 February 2021 1,009 5 View

How can I label an antibody to determine how far it is travelling from the microdialysis probe?

I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...

09 February 2021 3,981 1 View

How much of an effect does switching to a Whole foods plant based diet affect study and focus among college students?

I'm looking to write a proposal paper about the effect a whole foods plant based diet has on improving focus in studying and recall in testing among college students.

20 January 2021 323 2 View

Fluorophore panel selection?

Hello, I'm trying to select fluorophores for a large panel of flow cytometry, so I'm wondering what is the maximum degree of overlap between two fluorophores that is considered acceptable? Thanks.

19 January 2021 3,475 3 View

New/Creative Methods in Qualitative Research?

I will be conducting a qualitative research into well-being and help-seeking behaviour in elite coaching and I'm trying to think of new/creative ways how to do this rather than just conducting...

05 January 2021 5,158 4 View

How to develop mathematical model for Eye-Gaze tracking system?

Gaze tracking technologies are used in a wide variety of applications. Most gaze tracking systems require a personal calibration process where the user is instructed to gaze at certain points...

03 January 2021 9,675 5 View

Which is better to focus on teaching or research for a good academic life and Why?

Which is better to focus on teaching or research for For a good academic life and Why?

26 December 2020 5,737 9 View

What makes a strong research question?

A good research question is essential to guide your research paper, project, or thesis. It pinpoints exactly what you want to find out and gives your work a clear focus and purpose.

25 December 2020 6,145 4 View

How can I learn to read Zetasizer Diagrams professionally?

Hello all, I got this Zetasizer result recently and did a quick scanning here to learn how experts read these results. And end up with these data: 1.The most useful or serviceable info is the...

15 December 2020 6,658 5 View