Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

In meta-analysis of binary outcomes, what should we do when the occurrence of outcome is zero in both groups?

In conducting meta-analysis of binary outcomes (in RCTs), in case the risk ratio has not been reported, what should we do with the studies that the number of outcomes occurrence is zero in both...

09 January 2021 2,802 5 View

How to calculate ratio of means and ratio of geometric means?

In conducting a meta-analysis, sometimes, some studies have reported geometric mean, while others have reported arithmetic mean. One solution for pooling these studies is calculating the ratio of...

11 December 2020 4,663 3 View

Is (Are) there any valid corpus containing geographical events queries?

Hi all, I'm currently looking for a question corpus to have a plausible number of spatial events queries. I found MS MARCO dataset in which I could extract a reasonable number of geographical...

08 December 2020 7,844 2 View

Do I have to assume sphericity or not when conducting two way repeated measures ANOVA in graphpad prism 8?

I am analyzing data obtained from a crossover study conducted on same animals evaluating the effect of two different anesthetic drugs on heart rate, respiratory rate, pulse oximetry, rectal...

28 September 2020 6,408 4 View

Advices for: no amplification of aimed gene from transfected cells seleceted for it?

From a population of transfected/transduced cells I sort those with the desired expression. Starting from 10.000 cells I extract the ARN, and from its cDNA I amplify the gene of interest, as well...

19 September 2020 8,378 3 View

How to interpret arima model with regressors in r?

Hi there; I need to produce such an equation for a time series using arima model: y(t) = y(t-1) + a*x(t) + b*x(t-1) I know if I use for example arima (1,0,0) with x as the exogenous...

07 August 2020 7,438 6 View

Why some use seasonal ARIMA(p,d,q) (P,D, Q) instead of SARIMA?

Hello everyone. I am trying to use ARIMA model for a time series with seasonality. As I am new to this topic, I use the internet as my guide. My question is why anyone should use Seasonal ARIMA...

05 August 2020 219 4 View

How can I add stations to HBV-Light model?

Hi everyone. I've been using the original HBV model, so far. I might need to change it to HBV-light. Does anybody know how to add stations to HBV-light software? I can see there is this...

01 April 2020 1,032 7 View

How to define the parameters of Spatio-Temporal variogram models?

Hi everyone; I have a question regarding the parameters of variogram modelling. Does anyone know how to choose parameters such as Sill, Range and ... for the time, space and joint components of a...

10 February 2020 2,381 6 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

2D or 3D phased array antennas??

Dear All, I will start my PhD maybe this year regarding phased array antennas. I'm interested in to find more detailed information about 3D phased array antennas and 2D phased array antennas. Then...

21 February 2021 368 3 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View