How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Suggestions for using antisense oligonucleotide for brain delivery?

hello I am working on a project that includes gene delivery to the brain. my intentions are encapsulating a gene in a Chitosan NP and IV administration the NP to rats. beside plasmids is there...

29 January 2021 4,455 5 View

How to find degree of deacetylation of chitosan colorimetrically?

I am working with low DD% chitosan. It doesn't dissolve completely in acidic solutions. How to calculate the degree of deacetylation by potentiometric and acid-base titration, UV spectroscopic...

27 January 2021 8,532 2 View

How to calculate theoretical drug loading for polymer nanoparticle and calculated nanoparticle ?

nanoparticles coated with chitosan as well uncoated nanoparticle

26 January 2021 9,053 6 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

Do chemical and physical properties influence on the size of a polymer particle?

I would like to know if the properties of a polymer impact on the size of the particle it forms. I'm working with different polyssacharides (alginic acid from different seaweed), which are...

29 December 2020 7,333 6 View

Is the chitosan solution soluble in the physiological environment of PBS (pH: 6.2)?

Is the chitosan solution soluble in the physiological environment of PBS (pH: 6.2)?

27 December 2020 1,263 3 View

Can you suggest me how can I use D2O for dissolving chitosan sample for NMR analysis?

I am trying to dissolve chitosan with pure D2O but it is not dissolving. I read some articles that used D2O for dissolving chitosan before 1H and 13C NMR analysis? Do I need to do some...

14 December 2020 6,931 5 View

Is there a way to prevent agglomeration of chitosan when i add a chitosan solution dropwise to a bilosomes suspension for the purpose of coating?

I am doing research on the incorporation of bile salts into liposomes and coating the exterior surface with chitosan for enhanced stability in the GIT. Eveything goes well up until the point where...

14 December 2020 4,894 3 View

When increasing pH of a solution of chitosan (polycation), will the pKa increase or decrease?

The amino group has a pKa of ca. 6,5

09 December 2020 667 2 View