I am interested to study the microbial diversity of fish gut content. I am using QIAmp DNA stool mini kit for isolation of microbial DNA from fish gut contentas per manufacturers instruction, but I am getting degraded DNA. How to isolate long intact microbial DNA.
Hi Arjun,
I've attached a paper from our collaborators who work with fish gut microbiota. Take a look at the methods and maybe contact Kat Milligan-Myhre ([email protected]) if you have questions.
Good luck!
Danielle
QiAamp stool DNA kit does work well for any fecal samples but you can try MO-BIO power soil kit too for the same, but I think problem is different, try to separate the fecal content quickly from fresh fishes because pancreas and other organs contains lot of nucleases which might be derade the DNA.
There try to extract the fecal content from fishes which are still fresh. Hope helped.
HI I am facing problem with DNA extraction from fish gut samples. what is the method for inhibitor free DNA extraction that can be used for metagenomic analysis. Please suggest
QIAamp stool DNA kit contains Inhibitex tablets which give you clean DNA.
Hi Abhijit thank you for the reply. The DNA yield also very low with the methods I tried.
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