Arjun Sahoo

11 Questions 13 Answers 0 Followers

Questions related from Arjun Sahoo

Can anybody help me to run AfterQC for NGS sequence data quality check?

I have some Illumina sequence data. I want to check its quality using afterQC program but the problem is that AfterQC is running but no graphs are generating. Anybody could fix the problem.

09 September 2016 1,153 1 View

Is there any software that will help to present the read coverage in the form of graphs?Could anybody kindly help me to resolve the issue?

I am mapping the raw reads to the contigs generated by de novo assembly of the same reads and want to represent the read coverage in the form of graph..

09 September 2016 9,976 6 View

Could anybody help me to overcome the DNA isolation problem from fish gut content?

I am interested to study the microbial diversity of fish gut content. I am using QIAmp DNA stool mini kit for isolation of microbial DNA from fish gut contentas per manufacturers instruction, but...

05 May 2016 8,545 6 View

Difficulty in getting 5'RACE products

We are trying to get full cDNA using clontech's RACE kits (both 3' and 5' RACE) following the manufacturer's instruction for synthesis of 3' and 5' RACE but were not able to get the products....

02 February 2014 1,345 2 View

How to validate the putative SNPs? Which method is suitable for this purpose?

SNPs are abundant in the genome of any organism. We are in the process of SNP development through RNA-seq. We want to validate them by taking a panel of 48 unrelated individuals. The polymorphic...

08 August 2013 4,835 7 View

I am working in window based systems and want to load ubunto onto my PC. Can anyone kindly help me?

Since almost all the programs used for NGS data analysis are runing in linux environment, Linux (like Ubunto) is to be loaded in the PC.

05 May 2013 4,787 7 View

How to calculate sex specific recombination frequency?

I am using Joinmap version 4.1 for linkage analysis in F1 mapping population using microsatellite markers. While analysing I was not able to calculate sex specific recombination frequency...

04 April 2013 8,623 0 View

I am using an F1 mapping panel for linkage analysis

Microsatellites are the choice of markers in many non model organisms for linkage map construction. I am working on a non model fish species and developed around 140 informative markers in order...

02 February 2013 4,221 6 View

Construction of genetic map is based on recombination frequencies.

I am working on construction of genetic map of a fish species using LINKMFEX software. Could any body guide explain why recombination frequency differes in Male amd Female parents and how to...

09 September 2012 6,090 0 View

I wish to clone and characterize a gene in a species in which its sequence is not available. Can anyone suggest how I can proceed?

Since its sequence information is not available which method should I follow?

04 April 2012 2,212 4 View

Can any one please explain in detais about QTL mapping in F2 design?

Linkage based QTL mapping is one of the hot topic now adays.

11 November 2011 8,397 1 View