Is there any software that will help to present the read coverage in the form of graphs?Could anybody kindly help me to resolve the issue?

I am mapping the raw reads to the contigs generated by de novo assembly of the same reads and want to represent the read coverage in the form of graph..

08 September 2016 9,893 6 View

Can anybody help me to run AfterQC for NGS sequence data quality check?

08 September 2016 1,083 1 View

Could anybody help me to overcome the DNA isolation problem from fish gut content?

04 May 2016 8,466 6 View

Difficulty in getting 5'RACE products

We are trying to get full cDNA using clontech's RACE kits (both 3' and 5' RACE) following the manufacturer's instruction for synthesis of 3' and 5' RACE but were not able to get the products....

01 February 2014 1,286 2 View

How to validate the putative SNPs? Which method is suitable for this purpose?

SNPs are abundant in the genome of any organism. We are in the process of SNP development through RNA-seq. We want to validate them by taking a panel of 48 unrelated individuals. The polymorphic...

07 August 2013 4,732 7 View

I am working in window based systems and want to load ubunto onto my PC. Can anyone kindly help me?

Since almost all the programs used for NGS data analysis are runing in linux environment, Linux (like Ubunto) is to be loaded in the PC.

04 May 2013 4,720 7 View

How to calculate sex specific recombination frequency?

I am using Joinmap version 4.1 for linkage analysis in F1 mapping population using microsatellite markers. While analysing I was not able to calculate sex specific recombination frequency...

03 April 2013 8,523 0 View

I am using an F1 mapping panel for linkage analysis

Microsatellites are the choice of markers in many non model organisms for linkage map construction. I am working on a non model fish species and developed around 140 informative markers in order...

01 February 2013 4,146 6 View

Construction of genetic map is based on recombination frequencies.

I am working on construction of genetic map of a fish species using LINKMFEX software. Could any body guide explain why recombination frequency differes in Male amd Female parents and how to...

08 September 2012 6,023 0 View

I wish to clone and characterize a gene in a species in which its sequence is not available. Can anyone suggest how I can proceed?

Since its sequence information is not available which method should I follow?

03 April 2012 2,107 4 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

What is the role of fresh cow dung in sprouting in stem cutting propagation?

In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.

24 December 2020 4,068 6 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View

How to cut dsDNA chain with Uracil-DNA Glycosylase at the point of dU in room temperature?

18 December 2020 6,908 3 View

Why pink coloured or light red onion have more storage life than white and dark red one and smaller size one to has more than bigger one?

It is generally seen light coloured varieties has more storage life.what's relation with colour and size with storage life.

13 December 2020 634 18 View

Can LAMP distinguish the species from different genus/family?

I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...

07 December 2020 5,630 3 View