I am working on construction of genetic map of a fish species using LINKMFEX software. Could any body guide explain why recombination frequency differes in Male amd Female parents and how to detect these differences in LINKMFEX?
I am mapping the raw reads to the contigs generated by de novo assembly of the same reads and want to represent the read coverage in the form of graph..
08 September 2016 9,999 6 View
I have some Illumina sequence data. I want to check its quality using afterQC program but the problem is that AfterQC is running but no graphs are generating. Anybody could fix the problem.
08 September 2016 1,181 1 View
I am interested to study the microbial diversity of fish gut content. I am using QIAmp DNA stool mini kit for isolation of microbial DNA from fish gut contentas per manufacturers instruction, but...
04 May 2016 8,568 6 View
We are trying to get full cDNA using clontech's RACE kits (both 3' and 5' RACE) following the manufacturer's instruction for synthesis of 3' and 5' RACE but were not able to get the products....
01 February 2014 1,387 2 View
SNPs are abundant in the genome of any organism. We are in the process of SNP development through RNA-seq. We want to validate them by taking a panel of 48 unrelated individuals. The polymorphic...
07 August 2013 4,870 7 View
Since almost all the programs used for NGS data analysis are runing in linux environment, Linux (like Ubunto) is to be loaded in the PC.
04 May 2013 4,815 7 View
I am using Joinmap version 4.1 for linkage analysis in F1 mapping population using microsatellite markers. While analysing I was not able to calculate sex specific recombination frequency...
03 April 2013 8,650 0 View
Microsatellites are the choice of markers in many non model organisms for linkage map construction. I am working on a non model fish species and developed around 140 informative markers in order...
01 February 2013 4,247 6 View
Since its sequence information is not available which method should I follow?
03 April 2012 2,236 4 View
Linkage based QTL mapping is one of the hot topic now adays.
10 November 2011 8,416 1 View
is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?
05 July 2024 4,070 3 View
It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?
15 June 2024 1,355 7 View
I found a variant segregating with a genetic condition, this variant is listed in ClinVar (with no clinical significance), gnomAD, etc. However, I could not find any publication reporting the...
10 June 2024 7,606 3 View
Describe the principles of Population Genetics and evolution as applied to livestock breeding programs
08 June 2024 2,924 0 View
I used hygromycin and kanamycin for selecting positive buds in Micro-tom genetic transformation. Kanamycin performed very well and I could get positive plants soon, but hygromycin is a little bit...
29 May 2024 6,911 0 View
Hi, I've recently come across the colony of Geothrichum silvicola (based on sequencing result) isolated from soil with slimy morphology, unlike the colony morphology I've found on the journal...
13 May 2024 1,356 8 View
Hello I am currently engaged in research focusing on plant genetic transformation. As part of this endeavor, I have designed a comprehensive in silico plasmid cloning approach. The aim is to...
07 May 2024 5,119 3 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
Who (first) proposed/used/coined the term ‘translation’ in biology/genetics? What is the history behind the use of the word? Thank you!
01 May 2024 4,528 10 View
Can anyone assist with running the Linkage Analysis Tool 'Easylinkage' or any alternative tool for conducting linkage analysis and calculating LOD scores?
06 April 2024 7,273 2 View