I am using Joinmap version 4.1 for linkage analysis in F1 mapping population using microsatellite markers. While analysing I was not able to calculate sex specific recombination frequency difference. Could anyone help me?
I am mapping the raw reads to the contigs generated by de novo assembly of the same reads and want to represent the read coverage in the form of graph..
08 September 2016 9,893 6 View
08 September 2016 1,083 1 View
04 May 2016 8,466 6 View
We are trying to get full cDNA using clontech's RACE kits (both 3' and 5' RACE) following the manufacturer's instruction for synthesis of 3' and 5' RACE but were not able to get the products....
01 February 2014 1,286 2 View
SNPs are abundant in the genome of any organism. We are in the process of SNP development through RNA-seq. We want to validate them by taking a panel of 48 unrelated individuals. The polymorphic...
07 August 2013 4,732 7 View
Since almost all the programs used for NGS data analysis are runing in linux environment, Linux (like Ubunto) is to be loaded in the PC.
04 May 2013 4,720 7 View
Microsatellites are the choice of markers in many non model organisms for linkage map construction. I am working on a non model fish species and developed around 140 informative markers in order...
01 February 2013 4,146 6 View
I am working on construction of genetic map of a fish species using LINKMFEX software. Could any body guide explain why recombination frequency differes in Male amd Female parents and how to...
08 September 2012 6,023 0 View
Since its sequence information is not available which method should I follow?
03 April 2012 2,107 4 View
Linkage based QTL mapping is one of the hot topic now adays.
10 November 2011 8,325 1 View
Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...
21 February 2021 7,260 4 View
Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...
02 February 2021 2,613 7 View
Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...
02 February 2021 3,861 3 View
I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...
01 January 2021 1,580 4 View
I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...
31 December 2020 5,871 4 View
In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.
24 December 2020 4,068 6 View
Do you think the plant can communicate with each other, what is the level of feeling in plants?
22 December 2020 2,754 17 View
18 December 2020 6,908 3 View
It is generally seen light coloured varieties has more storage life.what's relation with colour and size with storage life.
13 December 2020 634 18 View
I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...
07 December 2020 5,630 3 View