I have some Illumina sequence data. I want to check its quality using afterQC program but the problem is that AfterQC is running but no graphs are generating. Anybody could fix the problem.
Thanks a lot
I am mapping the raw reads to the contigs generated by de novo assembly of the same reads and want to represent the read coverage in the form of graph..
08 September 2016 9,999 6 View
I am interested to study the microbial diversity of fish gut content. I am using QIAmp DNA stool mini kit for isolation of microbial DNA from fish gut contentas per manufacturers instruction, but...
04 May 2016 8,568 6 View
We are trying to get full cDNA using clontech's RACE kits (both 3' and 5' RACE) following the manufacturer's instruction for synthesis of 3' and 5' RACE but were not able to get the products....
01 February 2014 1,387 2 View
SNPs are abundant in the genome of any organism. We are in the process of SNP development through RNA-seq. We want to validate them by taking a panel of 48 unrelated individuals. The polymorphic...
07 August 2013 4,870 7 View
Since almost all the programs used for NGS data analysis are runing in linux environment, Linux (like Ubunto) is to be loaded in the PC.
04 May 2013 4,815 7 View
I am using Joinmap version 4.1 for linkage analysis in F1 mapping population using microsatellite markers. While analysing I was not able to calculate sex specific recombination frequency...
03 April 2013 8,650 0 View
Microsatellites are the choice of markers in many non model organisms for linkage map construction. I am working on a non model fish species and developed around 140 informative markers in order...
01 February 2013 4,247 6 View
I am working on construction of genetic map of a fish species using LINKMFEX software. Could any body guide explain why recombination frequency differes in Male amd Female parents and how to...
08 September 2012 6,116 0 View
Since its sequence information is not available which method should I follow?
03 April 2012 2,236 4 View
Linkage based QTL mapping is one of the hot topic now adays.
10 November 2011 8,416 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?
05 August 2024 8,836 2 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View