Is it possible to combine result of two apps in cytoscape?

11 October 2016 5,601 3 View

Lab Collaborators for Zika Virus Neurotropism and Pathogenesis?

06 April 2016 2,898 3 View

Can anyone refer me to a good heat map program for epitopes?

The data sheet produced affinities of 4800 consecutive segments of 15 amino acids, each overlapping by one single amino acid progressing downstream to cover the entire length of the 188 AA...

17 November 2015 6,473 2 View

Which commercially available anti-His antibody has the best performance for immunoblot/Western analyses of 6His-tagged proteins?

I have tried a few primary antibodies to detect His-tagged proteins on westerns, and been disappointed with the low signal intensity compared to other primary antibodies used in my lab. I would...

30 March 2015 3,558 8 View

Looking for help: What's the distinction between self-deception and self-concealment?

We (me and my research partner Thomas Waanders) have interviewed 20 Dutch Olympic Gold Medal winners about coping with extreme fatigue. At the moment we're analyzing the results and writing a book...

31 August 2014 5,672 12 View

Can anyone help me, I am having a difficult time trying to clone my small ~500bp gene sequence into pRU1101 & pRU1105 plasmids?

02 July 2014 2,964 3 View

How should I calculate a persons' years with standardized mortality ratios?

I am in the process of analyzing mortality statistics for those who come through the public behavioral health system in Ohio (e.g., severely mentally ill - SMI). To this end, I will be calculating...

29 August 2013 6,204 3 View

is the HCT116 colon cancer cell line homozygous for delta5-7 MRE11?

I'm trying to work out whether all HCT116 cells are positive for the 5-7 exon delete of MRE11 in the MRN complex

11 July 2013 1,580 2 View

To rake or not to rake, that is the question.

My colleagues and I conducted a study on the prevalence of gambling addiction in Ohio .The study was based on a household telephone survey of 3,600 Ohioans (18+) to get baseline prevalence data...

11 March 2013 8,639 7 View

At which stage do I add variability when performing multiple imputation?

09 November 2012 2,702 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View