For one of my internship projects I need to measure the difference in protein content from 2 oat samples. I am planning to use a bradford protein assay for this and in the protocol it says to dilute the protein BSA for the standard curve in the same buffer as the sample protein. What are some examples of protein dilution buffers i can use? And do I need to isolate the protein from the oats or can i just use grounded oats and a lysis buffer? In that case I would need around 11mg oats in 1 ml protein dilution buffer which should be doable. Thanks in advance!
Document Connect (thermofisher.com)
The ideal case is to use the same buffer in which the samples are dissolved. If the buffer contains nothing that would interfere in the assay (detergents or organic solvents, for example), it is OK to use distilled water.
See page 6 of this document for a list of interfering substances.
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011181_Coomassie_Bradford_Protein_Asy_UG.pdf
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