Rick Beardmore

3 Questions 2 Answers 0 Followers

Questions related from Rick Beardmore

Should you collect dead floating cells aswell and harvesting live cells when performing the Annexin V flow cytometry assay?

Trying to optimise the Annexin v -FIT-C kit for flow cytometry. 24 hours and especially 48 hours after drug treatment i'm getting very low viability with alot of dead floating debris but a...

06 November 2014 8,282 5 View

copGFP expression using a plate reader?

Following shRNA transfection, I'm trying to quantify the GFP expression using a plate reader. I know I have the right excitation/emission wavelength but I can't get it to work. Is the type of 96...

07 November 2013 2,232 1 View

is the HCT116 colon cancer cell line homozygous for delta5-7 MRE11?

I'm trying to work out whether all HCT116 cells are positive for the 5-7 exon delete of MRE11 in the MRN complex

12 July 2013 1,659 2 View