Conserved miRNA in plants?

More Magda Klimek-Ch's questions See All

Which Digital Elevation Model is better for Non point source pollution modelling?

Out of freely accessible Digital Elevation Model Which one is better suitable for Non-point source pollution modeling? (Indian terrain) I want to apply hydrological modeling for the study. The...

04 August 2020 8,204 3 View

In my study there are 1 independent, 5moderator and 3dependant, and only 1DV is correlated, should I perform regression and moderation for all ?

Only one dependent is correlated with independent, now regression and moderation analysis should run for all variable or only one variable which is corelated?

29 July 2020 4,470 6 View

Which website can provide inflow and outflow values for a reservoir in India?

Currently I am working on sedimentation assessment of Hirakud reservoir for this I need inflow and outflow values to calculate trap efficiency so can anyone suggest a website for retrieving these...

29 June 2020 6,530 1 View

What are the Challenges for Small Holder Farmers during Covid-times ?

Small holder agriculture will not be the same way during Covid period. Several challenges are faced by small holder farmers What the critical issues to be address for better farming in Small Holders

25 May 2020 7,129 34 View

How to define hyper elastic UMAT in a UEL?

Hi all, I am working on Phase-Field UEL for hyperelastic materials. I am using codes of Dr Molnar ( http://www.molnar-research.com/tutorials_videos_3.html) to develop hyperelastic material. I...

07 April 2020 4,243 1 View

How to do Openshell singlet calculations in Gaussian?

I have a system with a diradical character. I want to know whether it is triplet diradical or singlet diradical. How we can do that in gaussian?

31 March 2020 6,691 5 View

Hi, Could anybody suggest how can i determine the nature of FMOs of a molecule?

In detail, I want to know whether the HOMO of my molecule is either bonding or anti-bonding in nature? Please suggest me the software or code that can give the percentage of the antibonding...

15 March 2020 6,670 3 View

How much it costs for conducting hydrographic surveys on reservoir for storage estimation?

Currently, I am working on a Remote sensing-based approach for capacity estimation of a reservoir. for comparison purposes, I want to mention the expenditure of the hydrographic survey approach...

05 March 2020 1,634 8 View

What are latest machine learning technique for medical image analysis to compare with state of art?

What are latest machine learning technique for medical image analysis to compare with state of art

17 July 2019 6,228 9 View

Can someone suggest, best authors/professors/scholars for service recovery using justice theory?

Please suggest top 5 journals for service recovery?

13 February 2018 8,375 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

Ujjal Kumar Nath

The attach manuscript might be helpful for you to get your answer:

Magda Klimek-Ch

Ujjal Kumar Nath Thank you for your response. In the given article was the information that blastn against miRBase was made. Were any additional paramters were set? Can you recommend a specific software?

Ramamoorthy Sankaranarayanan

The following article deals about annotating miRNAs. For detail explanation please ping me.

http://www.plantcell.org/content/20/12/3186.long .

As Ujjal Kumar Nath mentioned, Blast against miRBase repository retrieve the reported miRNAs.

@ Ramamoorthy Sankaranarayanan Thank you for your answer. Should I allow for some mismatch during blastn analysis?

Yes as per Mayer's criteria

Great, thanks. I'm going to read this paper

Cheers

Have you gone through the article? Was that useful to you?

Hi, Thanks for this article. I found that there could be 2 mismatch nucleotide. Do you know a good software for that purpose?

Software for what purpose?

In my PhD, I have predicted and screened plant miRNAs. i am willing to help you. Please be specific in your questions so that I could understand and help you better.

OK :)

I have two multifasta files (1. - known plant miRNA from miRBase; 2. my miRNA). Now I want to search in first file similar seq (with 0, 1 or 2 mismatch) to check which my miRNA are known.