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Questions related from Ujjal Kumar Nath
In qPCR, I am using sometime 6 microL Syber with 1 miro cDNA, 2 micro F/R primers and 1 micro water in a 10 microL reaction mix together. I also use 12 micro Syber miro cDNA, 2 micro F/R primers...
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In qPCR, we normally use 45 to 50 cycles of for getting good amplification curve. However, I have some primers which start to get amplification raising peak after 50 cycles and still not complete...
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We are making cross between tetraploid and diploid water melon, but in the seed mixture we found some selfed seeds, that might be fusion of diploid and haploid female gamets within the egg sac....
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