whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?

whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?

07 September 2020 7,431 5 View

Has anyone had problem with single cell whole genome amplification?

Hello, I am experiencing problem with single cell whole genome amplification from 10-12 cells only 3 or 4 amplifies and I have a smear on gel electrophoresis, Can anyone help me? Best Regards

12 February 2018 3,212 5 View

Modeling Nucleate Boiling Using ANSYS FLUENT

20 June 2016 5,466 6 View

How should I use patch function for defining liquid-vapor interface?

Hi. I am working on a simulation of evaporation in a capillary tube.I am simulating evaporation of a water droplet sitting at the end of a capillary tube inside a solid body subjected to heat...

16 June 2016 508 16 View

Can I do with COMSOL simulation of intense laser plasma interaction and I want to expose this laser-produced plasma in an elecric field?

I need COMSOL code for simulation of intense laser plasma interaction and I want to expose this laser-produced plasma in an electric field to see double layering and then seed this laser-produced...

03 April 2016 1,597 2 View

How can I remove the staining from the background in IHC?

I am doing IHC using an ABC staining kit (centacruz), concentration of primary and secondary antibody is optimized to detect the desired protein, incubate DAB 30 min, counterstain 5 second, but...

08 March 2013 3,561 2 View

What is the best way to increase the concentration of proteins?

I extracted total proteins from tissue to do western blot. Some of my samples are in low concentration. Since I need to run equal proteins for SDS page, I want to increase the concentration of...

08 February 2013 1,857 25 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View