whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?

whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?

07 September 2020 7,431 5 View

Has anyone had problem with single cell whole genome amplification?

Hello, I am experiencing problem with single cell whole genome amplification from 10-12 cells only 3 or 4 amplifies and I have a smear on gel electrophoresis, Can anyone help me? Best Regards

12 February 2018 3,212 5 View

Modeling Nucleate Boiling Using ANSYS FLUENT

20 June 2016 5,466 6 View

How should I use patch function for defining liquid-vapor interface?

Hi. I am working on a simulation of evaporation in a capillary tube.I am simulating evaporation of a water droplet sitting at the end of a capillary tube inside a solid body subjected to heat...

16 June 2016 508 16 View

Can I do with COMSOL simulation of intense laser plasma interaction and I want to expose this laser-produced plasma in an elecric field?

I need COMSOL code for simulation of intense laser plasma interaction and I want to expose this laser-produced plasma in an electric field to see double layering and then seed this laser-produced...

03 April 2016 1,597 2 View

How can I remove the staining from the background in IHC?

I am doing IHC using an ABC staining kit (centacruz), concentration of primary and secondary antibody is optimized to detect the desired protein, incubate DAB 30 min, counterstain 5 second, but...

08 March 2013 3,561 2 View

What is the best way to increase the concentration of proteins?

I extracted total proteins from tissue to do western blot. Some of my samples are in low concentration. Since I need to run equal proteins for SDS page, I want to increase the concentration of...

08 February 2013 1,857 25 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How to obtain parameters to run a simulation for benzene using CHARMM forcefield in GROMACS?

I have been reading this paper Soft Matter, 2017, 13, 1904 and in this paper, they simulate benzene in GROMACS using CHARMM. They mention that they performed simulations of benzene using the...

15 February 2021 5,561 3 View

Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

I have some problems with the mass transfer part of the CFD simulation of membrane distillation.Can anyone help me?

I am working on modeling of Direct Contact Membrane Distillation system with COMSOL Multiphysics software. I have some problems with the mass transfer part of the simulation because I don't know...

14 February 2021 379 2 View